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首页> 外文期刊>Journal of Molecular Biology >MUTATIONAL ANALYSIS OF THE SEQUENCE-SPECIFIC RECOMBINATION BOX FOR AMPLIFICATION OF GENE 17 OF BACTERIOPHAGE T4
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MUTATIONAL ANALYSIS OF THE SEQUENCE-SPECIFIC RECOMBINATION BOX FOR AMPLIFICATION OF GENE 17 OF BACTERIOPHAGE T4

机译：扩增噬菌体T4基因17的序列特异性重组盒的突变分析

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摘要

Bacteriophage T4 gene 17 amplification mutants Hp17 that carry two to six tandem repeats of the genes 17-18 region were isolated by growth of gene 17 amber mutants on ochre suppressor strains of Escherichia coli. These mutants arise from an initial sequence-specific recombination between two GCTCA sequences in a 24 bp imperfect homology box in genes 16 and 19. The initial recombination occurred in the wild-type phage T4 population, as shown by polymerase chain reaction, at a frequency of about 10(-6), which is consistent with the frequency of mutant isolation. T4 phage with mutations of the 3rd, 6th, 9th, 12th, or 15th positions in the 24 bp box of gene 16 either failed to produce gene amplification mutant Hp17 or produced gene amplification mutants from an initial recombination at other regions. Among the mutants that failed to produce gene amplification mutants, the initial recombination generally occurred at lower frequencies at either the GCTCA sequence or other sequences. Since the gene amplification mutations are eliminated or shifted to different sequences by base changes that increase as well as decrease homology, the predominant recombination event between the gene 16 and 19 recombination boxes appears to be sequence-dependent rather than homology-dependent. [References: 50]
机译：携带噬菌体T4基因17扩增突变体Hp17，通过在大肠杆菌的region色抑制菌株中生长基因17琥珀突变体，分离出携带基因17-18区域的2至6个串联重复序列。这些突变体来自基因16和19中24 bp不完全同源框中两个GCTCA序列之间的初始序列特异性重组。如聚合酶链式反应所示，该初始重组发生在野生型噬菌体T4群体中，频率为大约10（-6），这与突变体分离的频率一致。在基因16的24 bp框中具有第3，第6，第9，第12或第15位突变的T4噬菌体无法产生基因扩增突变体Hp17或无法通过其他区域的初始重组产生基因扩增突变体。在未能产生基因扩增突变体的突变体中，初始重组通常在GCTCA序列或其他序列上以较低的频率发生。由于基因扩增突变通过增加和减少同源性的碱基改变而被消除或转移到不同的序列，因此基因16和19重组盒之间的主要重组事件似乎是序列依赖性的，而不是同源性依赖性的。 [参考：50]

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