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Direct visualization of extensibility in isolated titin molecules

机译:直接可视化分离的替丁分子的可扩展性

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"Molecular combing" induced by a receding meniscus has been shown to extend individual titin molecules. Electron microscopy reveals that both ends of the molecule tend to attach to a mica substrate, probably due to their local positive charges. This leaves the remainder of the molecule free to be straightened and extended by forces of up to approximately 800 pN. A small region in the I-band part of the molecule, which probably corresponds to sequence high in P, E, V and K residues, is the most compliant and appears to extend by an unfolding of the polypeptide chain. Other parts of the molecule are also capable of extension. These mechanical extensions in titin are probably reversible.
机译:半月板后退引起的“分子梳理”已显示出可扩展单个肌动蛋白分子。电子显微镜显示分子的两端倾向于附着在云母基底上,这可能是由于它们的局部正电荷所致。这使得分子的其余部分可以自由地通过高达约800 pN的力进行矫直和延伸。分子的I带部分中的一个小区域,可能与P,E,V和K残基较高的序列相对应,是最顺应性的,并且似乎通过多肽链的展开而延伸。分子的其他部分也能够延伸。肌动蛋白的这些机械延伸可能是可逆的。

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