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首页> 外文期刊>Journal of Molecular Biology >ANALYSES OF CARBOHYDRATE RECOGNITION BY LEGUME LECTINS - SIZE OF THE COMBINING SITE LOOPS AND THEIR PRIMARY SPECIFICITY
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ANALYSES OF CARBOHYDRATE RECOGNITION BY LEGUME LECTINS - SIZE OF THE COMBINING SITE LOOPS AND THEIR PRIMARY SPECIFICITY

机译：豆类凝集素对碳水化合物的识别-结合环的大小及其主要特异性。

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Recognition of cell-surface carbohydrates by lectins has wide implications in Important biological processes. The ability of plant lectins to detect subtle variations in carbohydrate structures found on molecules, cells and organisms have made them a paradigm for protein-carbohydrate recognition. Legume lectins, one of the most well studied family of plant proteins, display a considerable repertoire of carbohydrate specificities owing perhaps to the sequence hypervariability in the loops constituting their combining site. However, lack of a rigorous framework to explain their carbohydrate binding specificities has precluded a rational approach to alter their ligand binding activity in a meaningful manner. This study reports an extensive analysis of sequences and structures of several legume lectins and shows that despite the hypervariability of their combining regions they exhibit within a significant pattern of uniformity. The results show that the size of the binding site loop D is invariant in the Man/Glc specific lectins and is possibly a primary determinant of the monosaccharide specificities of the legume lectins. Analyses of size and sequence variability of loops reveal the existence of a common theme that subserves to define their binding specificities. These results thus provide not only a framework for understanding the molecular basis of carbohydrate recognition by legume lectins but also a rationale for redesign of their ligand binding propensities. (C) 1997 Academic Press Limited. [References: 64]
机译：凝集素对细胞表面碳水化合物的识别在重要的生物过程中具有广泛的意义。植物凝集素检测分子，细胞和生物体中发现的碳水化合物结构细微变化的能力使其成为蛋白质-碳水化合物识别的范例。豆科植物凝集素是研究最深入的植物蛋白家族之一，显示出相当大的碳水化合物特异性，这可能是由于构成其结合位点的环中的序列具有高变异性。然而，由于缺乏严格的框架来解释它们的碳水化合物结合特异性，因此无法采取合理的方法以有意义的方式改变其配体结合活性。这项研究报告了对几种豆科植物凝集素的序列和结构的广泛分析，结果表明，尽管它们的结合区具有高度可变性，但它们仍表现出明显的均匀性。结果表明，结合位点环D的大小在Man / Glc特异性凝集素中是不变的，并且可能是豆类凝集素的单糖特异性的主要决定因素。对环的大小和序列变异性的分析揭示了存在一个共同的主题，该主题可用于定义其结合特异性。因此，这些结果不仅为理解豆科植物凝集素识别碳水化合物的分子基础提供了框架，而且为重新设计其配体结合倾向提供了理论依据。 （C）1997 Academic Press Limited。 [参考：64]

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