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首页> 外文期刊>Journal of Molecular Biology >The 9 A projection structure of cytochrome b6f complex determined by electron crystallography.
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The 9 A projection structure of cytochrome b6f complex determined by electron crystallography.

机译：通过电子晶体学测定的细胞色素b6f复合物的9 A投影结构。

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摘要

Thin three-dimensional crystals of the cytochrome b6 f complex from the unicellular algae Chlamydomonas reinhardtii have been grown by BioBeads-mediated detergent removal from a mixture of protein and lipid solubilized in Hecameg. Frozen-hydrated crystals, exhibiting p22121 plane group symmetry, were studied by electron crystallography and a projection map at 9 A resolution was calculated. The crystals (unit cell dimensions of a=173.5 A, b=70.0 A and gamma=90.0 degrees) showed the presence of dimers, and within each monomer 14 domains of electron density were observed. The combination of the projection map obtained from ice-embedded crystals of cytochrome b6 f with a previous map obtained from negatively stained samples brings new insight in the organization of the complex. For example, it distinguishes some peaks and/or domains that are only extramembrane or transmembrane, and reveals the possible localization of single-stranded transmembrane alpha-helices (Pet subunits). Furthermore, the cross-correlation of our projection map from frozen hydrated samples with the atomic model of the transmembrane part of the cytochrome bc1 complex has allowed us to localize the cytochrome b6 at the dimer interface and to reveal structural differences between the two complexes. Copyright 1999 Academic Press.
机译：来自单细胞藻类莱茵衣藻的细胞色素b6f复合物的薄三维晶体已经通过BioBeads介导的去垢剂从Hecameg中溶解的蛋白质和脂质的混合物中去除而得到了生长。通过电子晶体学研究了具有p22121平面群对称性的冷冻水合晶体，并计算了9 A分辨率的投影图。晶体（a ＝ 173.5A，b ＝ 70.0A，γ＝ 90.0度的晶胞尺寸）显示二聚体的存在，并且在每个单体内观察到14个电子密度域。从细胞色素b6f的冰包埋晶体获得的投影图与从负染样品获得的先前图的组合为复合物的组织提供了新的见识。例如，它区分了仅是膜外或跨膜的一些峰和/或域，并揭示了单链跨膜α-螺旋（Pet亚基）的可能定位。此外，我们的冷冻水合样品投影图与细胞色素bc1复合物跨膜部分的原子模型的互相关性使我们能够将细胞色素b6定位在二聚体界面处，并揭示了两种复合物之间的结构差异。版权所有1999，学术出版社。

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