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首页> 外文期刊>Journal of Molecular Biology >The 23 S rRNA environment of ribosomal protein L9 in the 50 S ribosomal subunit.
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The 23 S rRNA environment of ribosomal protein L9 in the 50 S ribosomal subunit.

机译：50 S核糖体亚基中核糖体蛋白L9的23 S rRNA环境。

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摘要

Ribosomal protein L9 consists of two globular alpha/beta domains separated by a nine-turn alpha-helix. We examined the rRNA environment of L9 by chemical footprinting and directed hydroxyl radical probing. We reconstituted L9, or individual domains of L9, with L9-deficient 50 S subunits, or with deproteinized 23 S rRNA. A footprint was identified in domain V of 23 S rRNA that was mainly attributable to N-domain binding. Fe(II) was tethered to L9 via cysteine residues introduced at positions along the alpha-helix and in the C-domain, and derivatized proteins were reconstituted with L9-deficient subunits. Directed hydroxyl radical probing targeted regions of domains I, III, IV, and V of 23 S rRNA, reinforcing the view that 50 S subunit architecture is typified by interwoven rRNA domains. There was a striking correlation between the cleavage patterns from the Fe(II) probes attached to the alpha-helix and their predicted orientations, constraining both the position and orientation of L9, as well as the arrangement of specific elements of 23 S rRNA, in the 50 S subunit. Copyright 2000 Academic Press.
机译：核糖体蛋白L9由两个球形的alpha / beta结构域组成，这些结构域由九圈的alpha-螺旋隔开。我们通过化学足迹法和定向羟基自由基探针研究了L9的rRNA环境。我们用L9缺失的50 S亚基或去蛋白的23 S rRNA重建L9或L9的单个结构域。在23S rRNA的结构域V中鉴定出足迹，其主要归因于N结构域结合。 Fe（II）通过沿α-螺旋和C结构域引入的半胱氨酸残基束缚在L9上，衍生的蛋白质被L9缺失的亚基重建。指导羟基自由基探测23 S rRNA的结构域I，III，IV和V的目标区域，强化了这样的观点，即50 S亚基结构以交织的rRNA结构域为代表。在连接到α-螺旋的Fe（II）探针的切割模式与它们的预测方向之间存在惊人的相关性，不仅限制了L9的位置和方向，还限制了23 S rRNA的特定元素的排列。 50 S亚单位。版权所有2000学术出版社。

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