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首页> 外文期刊>Journal of Molecular Biology >ANALYSIS OF ACTION OF THE WOBBLE ADENINE ON CODON READING WITHIN THE RIBOSOME
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ANALYSIS OF ACTION OF THE WOBBLE ADENINE ON CODON READING WITHIN THE RIBOSOME

机译：核糖体中可疑腺嘌呤对密码子阅读的作用分析

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Computer graphics simulation of the interaction between the codon-anticodon duplexes containing adenine in the first (wobble) position of the anticodons, and bound to the ribosomal A- and P-sites, was made. This demonstrated that widespread use of adenine in the wobble position in anticodons should lead to a low efficiency of ribosomal translation, since the wobble A of the P-site tRNA weakens the codon-dependent binding of aminoacyl-tRNA at the A-site via interduplex interaction. Besides the canonical partner U, the wobble A of aminoacyl-tRNA can recognize A, C, G in the third position of the codon by the formation of the propeller twist in the wobble pairs AA, AC, AG. The conversion of the wobble A into inosine improves its pairing with the codon bases (the pairs IA and IC, unlike AA and AC, should not form the propeller twist leading to the deformation of base-base hydrogen bonds) and should reduce an adverse effect of the P-site wobble adenine on the formation of the A-site duplex. The consequence of the interaction between the ribosomal P- and E-site duplexes has been formulated. According to this the E-site wobble A should enhance the probability of frameshifting. These properties of the wobble A and I could be a reason why A is very rarely observed in the first anticodon position and why evolutionary processes have developed the enzyme which modifies the wobble A to I. The results obtained can be subjected to direct experimental tests. (C) 1995 Academic Press Limited [References: 31]
机译：进行了计算机图形学模拟，在反密码子的第一个（摆动）位置包含腺嘌呤并与核糖体A和P位点结合的密码子-反密码子双链体之间的相互作用。这表明腺嘌呤在反密码子的摆动位置上的广泛使用应导致核糖体翻译的效率低下，因为P位点tRNA的摆动A减弱了通过双链体在A位点处的氨酰tRNA的密码子依赖性结合。相互作用。除了规范伴侣U，氨酰基tRNA的摆动A可以通过在摆动对AA，AC，AG中形成螺旋桨扭曲来识别密码子第三位置的A，C，G。摆动A向肌苷的转化改善了其与密码子碱基的配对（IA和IC对，与AA和AC不同，不应形成螺旋桨扭曲，导致碱基-氢键变形）并应减少不利影响位点摆动腺嘌呤对A位点双链体形成的影响。已经确定了核糖体P位和E位双链体之间相互作用的结果。据此，E位置摆动A应该增加移码的可能性。摆动A和I的这些特性可能是为什么在第一个反密码子位置很少观察到A以及为什么进化过程已经开发出将摆动A修饰为I的酶的原因。获得的结果可以直接进行实验测试。 （C）1995 Academic Press Limited [参考号：31]

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