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首页> 外文期刊>Journal of Molecular Biology >New structural insights into the molecular deciphering of mycobacterial lipoglycan binding to C-type lectins: lipoarabinomannan glycoform characterization and quantification by capillary electrophoresis at the subnanomole level.
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New structural insights into the molecular deciphering of mycobacterial lipoglycan binding to C-type lectins: lipoarabinomannan glycoform characterization and quantification by capillary electrophoresis at the subnanomole level.

机译：对分枝杆菌脂聚糖与C型凝集素结合的分子破译的新结构见解：脂阿拉伯甘露聚糖糖型的表征和在亚纳摩尔水平的毛细管电泳定量分析。

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摘要

Lipoarabinomannans are key molecules of the mycobacterial envelopes involved in many steps of tuberculosis immunopathogenesis. Several of the biological activities of lipoarabinomannans are mediated by their ability to bind human C-type lectins, such as the macrophage mannose receptor, the mannose-binding protein and the surfactant proteins A and D. The lipoarabinomannan mannooligosaccharide caps have been demonstrated to be involved in the binding to the lectin carbohydrate recognition domains. We report an original analytical approach, based on capillary electrophoresis monitored by laser-induced fluorescence, allowing the absolute quantification, in nanomole quantities of lipoarabinomannan, of the number of mannooligosaccharide units per lipoarabinomannan molecule. Moreover, this analytical approach was successful for the glycosidic linkage determination of the mannooligosaccharide motifs and has been applied to the comparative analysis of parietal and cellular lipoarabinomannans of Mycobacterium bovis BCG and Mycobacterium tuberculosis H37Rv, H37Ra and Erdman strains. Significant differences were observed in the amounts of the various mannooligosaccharide units between lipoarabinomannans of different strains and between parietal and cellular lipoarabinomannans of the same strain. Nevertheless, no relationship was found between the number of mannooligosaccharide caps and the virulence of the corresponding strain. The results of the present study should help us to gain more understanding of the molecular basis of lipoarabinomannan discrimination in the process of binding to C-type lectins. Copyright 2000 Academic Press.
机译：脂阿拉伯糖甘露聚糖是分枝杆菌包膜的关键分子，参与结核病免疫发病的许多步骤。脂阿拉伯糖甘露聚糖结合人C型凝集素的能力介导了几种生物活性，例如巨噬细胞甘露糖受体，甘露糖结合蛋白和表面活性剂蛋白A和D。已证明脂阿拉伯糖甘露聚糖甘露寡糖帽参与其中与凝集素碳水化合物识别域结合。我们报告了一种原始的分析方法，该方法基于通过激光诱导的荧光监测的毛细管电泳，可以绝对定量地定量每个脂阿拉伯甘露聚糖分子中甘露寡糖单位的纳摩尔量（以纳摩尔量计）。此外，这种分析方法成功地确定了甘露寡糖基序的糖苷键，并已用于牛分枝杆菌BCG和结核分枝杆菌H37Rv，H37Ra和Erdman菌株的壁和细胞脂阿拉伯甘露聚糖的比较分析。在不同菌株的脂阿拉伯甘露聚糖之间以及同一菌株的顶叶和细胞脂阿拉伯甘露聚糖之间的各种甘露寡糖单位的量上观察到显着差异。然而，在甘露寡糖帽的数目和相应菌株的毒力之间没有发现任何关系。本研究的结果应有助于我们更多地了解与C型凝集素结合过程中脂阿拉伯甘露聚糖区分的分子基础。版权所有2000学术出版社。

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