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首页> 外文期刊>Journal of Molecular Biology >Design and evolution of artificial M13 coat proteins.
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Design and evolution of artificial M13 coat proteins.

机译：人工M13外壳蛋白的设计和进化。

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Using simple design and selective pressure, we have evolved an artificial M13 bacteriophage coat protein. M13 coat proteins first reside in the bacterial inner membrane and subsequently surround the DNA core of the assembled virus. The artificial coat protein (ACP) was designed and evolved to mimic both functions of the natural M13 coat proteins, but with an inverted orientation. ACP is a non-functional coat protein because it is not required for the production of phage particles. Instead, it incorporates into a phage coat which still requires all the natural coat proteins for structural integrity. In contrast with other M13 coat proteins, which can display polypeptides as amino-terminal fusions, ACP permits the carboxy-terminal display of large polypeptides. The results suggest that viruses can co-opt host membrane proteins to acquire new coat proteins and thus new functions. In particular, M13 bacteriophage can be engineered for new functions, such as carboxy-terminal phage display. Copyright 2000 Academic Press.
机译：使用简单的设计和选择性的压力，我们已经进化出一种人工M13噬菌体外壳蛋白。 M13外壳蛋白首先驻留在细菌内膜中，然后围绕组装的病毒的DNA核心。设计并进化了人工外壳蛋白（ACP），以模仿天然M13外壳蛋白的两种功能，但方向相反。 ACP是一种无功能的外壳蛋白，因为它不是生产噬菌体颗粒所必需的。取而代之的是，它掺入了噬菌体外壳，该外壳仍然需要所有天然外壳蛋白来保证结构完整性。与其他M13外壳蛋白可以将多肽显示为氨基末端融合体相反，ACP允许大多肽的羧基末端显示。结果表明，病毒可以选择宿主膜蛋白来获得新的外壳蛋白，从而获得新的功能。特别地，可以将M13噬菌体工程化以具有新功能，例如羧基末端噬菌体展示。版权所有2000学术出版社。

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