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首页> 外文期刊>Journal of Molecular Biology >An expressed sequence tag (EST) data mining strategy succeeding in thediscovery of new G-protein coupled receptors
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An expressed sequence tag (EST) data mining strategy succeeding in thediscovery of new G-protein coupled receptors

机译：表达序列标签（EST）数据挖掘策略成功发现了新的G蛋白偶联受体

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摘要

We have developed a comprehensive expressed sequence tag database search method and used it for the identification of new members of the G-protein coupled receptor superfamily. Our approach proved to be especially useful for the detection of expressed sequence tag sequences that do not encode conserved parts of a protein, making it an ideal tool for the identification of members of divergent protein families or of protein parts without conserved domain structures in the expressed sequence tag database. At least 14 of the expressed sequence tags found with this strategy are promising candidates for new putative G-protein coupled receptors. Here, we describe the sequence and expression analysis of five new members of this receptor superfamily, namely GPR84, GPR86, GPR87, GPR90 and GPR91. We also studied the genomic structure and chromosomal localization of the respective genes applying in silico methods. A cluster of six closely related C-protein coupled receptors was found on the human chromosome 3q24-3q25, Tt consists of four orphan receptors (GPR86, GPR87, GPR91, and H963), the purinergic receptor P2Y1, and the uridine 5'-diphosphoglucose receptor KIAA0001. it seems likely that these receptors evolved from a common ancestor and therefore might have related ligands. In conclusion, we describe a data mining procedure that proved to be useful for the identification and first characterization of new genes and is well applicable for other gene Families.
机译：我们已经开发了一种全面的表达序列标签数据库搜索方法，并将其用于鉴定G蛋白偶联受体超家族的新成员。我们的方法被证明对检测不编码蛋白质保守部分的表达序列标签序列特别有用，使其成为鉴定差异蛋白家族成员或表达中没有保守域结构的蛋白质部分的理想工具序列标签数据库。用这种策略发现的至少14个表达的序列标签是新的假定的G蛋白偶联受体的有希望的候选者。在这里，我们描述了该受体超家族的五个新成员，即GPR84，GPR86，GPR87，GPR90和GPR91的序列和表达分析。我们还使用计算机方法研究了各个基因的基因组结构和染色体定位。在人类染色体3q24-3q25上发现了六个紧密相关的C蛋白偶联受体簇，Tt由四个孤儿受体（GPR86，GPR87，GPR91和H963），嘌呤能受体P2Y1和尿苷5'-二磷酸葡萄糖组成受体KIAA0001。这些受体似乎是从共同祖先进化而来的，因此可能具有相关的配体。总之，我们描述了一种数据挖掘程序，该程序被证明对新基因的识别和首次表征非常有用，并且非常适用于其他基因家族。

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