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首页> 外文期刊>Journal of Molecular Biology >Three-dimensional structure of a mammalian purple acid phosphatase at 2.2 A resolution with a mu-(hydr)oxo bridged di-iron center.
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Three-dimensional structure of a mammalian purple acid phosphatase at 2.2 A resolution with a mu-(hydr)oxo bridged di-iron center.

机译：哺乳动物紫色酸性磷酸酶的三维结构，分辨率为2.2 A，具有mu-（hydr）oxo桥接的二铁中心。

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摘要

The crystal structure of purple acid phosphatase from rat bone has been determined by molecular replacement and the structure has been refined to 2.2 A resolution to an R -factor of 21.3 % (R -free 26.5 %). The core of the enzyme consists of two seven-stranded mixed beta-sheets, with each sheet flanked by solvent-exposed alpha-helices on one side. The two sheets pack towards each other forming a beta-sandwich. The di-iron center, located at the bottom of the active-site pocket at one edge of the beta-sandwich, contains a mu-hydroxo or mu-oxo bridge and both metal ions are observed in an almost perfect octahedral coordination geometry. The electron density map indicates that a mu-(hydr)oxo bridge is found in the metal center and that at least one solvent molecule is located in the first coordination sphere of one of the metal ions. The crystallographic study of rat purple acid phosphatase reveals that the mammalian enzymes are very similar in overall structure to the plant enzymes in spite of only 18 % overall sequence identity. In particular, coordination and geometry of the iron cluster is preserved in both enzymes and comparison of the active-sites suggests a common mechanism for the mammalian and plant enzymes. However, significant differences are found in the architecture of the substrate binding pocket. Copyright 1999 Academic Press.
机译：已通过分子置换确定了来自大鼠骨骼的紫色酸性磷酸酶的晶体结构，并将该结构精制至2.2 A分辨率，R因子为21.3％（无R的26.5％）。酶的核心由两个七链混合的β-折叠组成，每个折叠的侧面都在一侧暴露有溶剂。两张纸彼此相对排列，形成β三明治。双铁中心位于β-三明治的一个边缘的活性位点口袋的底部，包含一个mu-hydroxo或mu-oxo桥，并且两种金属离子都以几乎完美的八面体配位几何结构被观察到。电子密度图表明在金属中心发现了一个mu-（羟基）氧代桥，并且至少一个溶剂分子位于一个金属离子的第一配位球中。对大鼠紫色酸性磷酸酶的晶体学研究表明，尽管整个序列同一性仅为18％，但哺乳动物酶的总体结构与植物酶非常相似。特别是，铁簇的协调性和几何形状在两种酶中均得到保留，并且对活性位点的比较提示了哺乳动物和植物酶的共同机制。但是，在底物结合袋的结构上发现了显着差异。版权所有1999，学术出版社。

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