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首页> 外文期刊>Journal of Molecular Biology >The Bacillus subtilis bacteriophage SPP1 G39P delivers and activates the G40P DNA helicase upon interacting with the G38P-bound replication origin.
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The Bacillus subtilis bacteriophage SPP1 G39P delivers and activates the G40P DNA helicase upon interacting with the G38P-bound replication origin.

机译：枯草芽孢杆菌噬菌体SPP1 G39P在与G38P结合的复制起点相互作用后传递并激活G40P DNA解旋酶。

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摘要

Initiation of Bacillus subtilis bacteriophage SPP1 replication requires the phage-encoded genes 38, 39 and 40 products (G38P, G39P and G40P). G39P, which does not bind DNA, interacts with the replisome organiser, G38P, in the absence of ATP and with the ATP-activated hexameric replication fork helicase, G40P. G38P, which specifically interacts with the phage replication origin (oriL) DNA, does not seem to form a stable complex with G40P in solution. G39P when complexed with G40P-ATP inactivates the single-stranded DNA binding, ATPase and unwinding activities of G40P, and such effects are reversed by increasing amounts of G38P. Unwinding of a forked substrate by G40P-ATP is increased about tenfold by the addition of G38P and G39P to the reaction mixture. The specific protein-protein interactions between oriL-bound G38P and the G39P-G40P-ATPgammaS complex are necessary for helicase delivery to the SPP1 replication origin. Formation of G38P-G39P heterodimers releases G40P-ATPgammaS from the unstable oriL-G38P-G39P-G40P-ATPgammaS intermediate. G40P-ATPgammaS binds to the origin region, the uncomplexed G38P fraction remains bound to oriL, and the G38P-G39P heterodimer is lost from the complex. We demonstrate that G39P is a component of an oligomeric nucleoprotein complex which plays an important role in the initiation of SPP1 replication. Copyright 1999 Academic Press.
机译：枯草芽孢杆菌噬菌体SPP1复制的启动需要噬菌体编码的基因38、39和40产物（G38P，G39P和G40P）。不结合DNA的G39P在不存在ATP的情况下与复制子组织器G38P相互作用，并与ATP激活的六聚体复制叉解旋酶G40P相互作用。与噬菌体复制起点（oriL）DNA特异性相互作用的G38P在溶液中似乎未与G40P形成稳定的复合物。当与G40P-ATP复合时，G39P可以使G40P的单链DNA结合，ATPase和解链活性失活，并且通过增加G38P的量可以逆转这种影响。通过向反应混合物中加入G38P和G39P，G40P-ATP对叉状底物的退绕增加了约十倍。 oriL结合的G38P和G39P-G40P-ATPgammaS复合体之间的特定蛋白质相互作用是将解旋酶递送至SPP1复制起点所必需的。 G38P-G39P异二聚体的形成从不稳定的oriL-G38P-G39P-G40P-ATPgammaS中间体释放G40P-ATPgammaS。 G40P-ATPgammaS结合到起始区域，未复合的G38P馏分仍与oriL结合，G38P-G39P异二聚体从复合物中丢失。我们证明G39P是寡聚核蛋白复合物的组成部分，它在SPP1复制的起始中起重要作用。版权所有1999，学术出版社。

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