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首页> 外文期刊>Journal of Molecular Biology >Crystal structure of an adenine bulge in the RNA chain of a DNA.RNA hybrid, d(CTCCTCTTC).r(gaagagagag).
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Crystal structure of an adenine bulge in the RNA chain of a DNA.RNA hybrid, d(CTCCTCTTC).r(gaagagagag).

机译：DNA.RNA杂交体d（CTCCTCTTC）.r（gaagagagag）的RNA链中腺嘌呤凸起的晶体结构。

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摘要

Crystal structure of a DNA.RNA hybrid, d(CTCCTCTTC).r(gaagagagag), with an adenine bulge in the polypurine RNA strand was determined at 2.3 A resolution. The structure was solved by the molecular replacement method and refined to a final R-factor of 19.9% (Rfree 22.2%). The hybrid duplex crystallized in the space group I222 with unit cell dimensions, a = 46.66 A, b = 47.61 A and c = 54.05 A, and adopts the A-form conformation. All RNA and DNA sugars are in the C3'-endo conformation, the glycosyl angles in anti conformation and the majority of the C4'-C5' torsion angles in g+ except two trans angles, in conformity with the C3'-endo rigid nucleotide hypothesis. The adenine bulge is looped out and it is also in the anti C3'-endo conformation. The bulge is involved in a base-triple (C.g)*a interaction with the end base-pair (C9.g10) in the minor groove of a symmetry-related molecule. The 2' hydroxyl group of g15 is hydrogen bonded to O2P and O5' of g17, skipping the bulged adenine a16 and stabilizing the sugar-phosphate backbone of the hybrid. The hydrogen bonding and the backbone conformation at the bulged adenine site is very similar to that found in the crystal structure of a protein-RNA complex.
机译：确定DNA.RNA杂种d（CTCCTCTTC）.r（gaagagagag）在多嘌呤RNA链​​中带有腺嘌呤凸起的晶体结构，分辨率为2.3A。通过分子置换方法解析结构，并将其精炼至最终R因子19.9％（Rfree 22.2％）。杂化双链体在晶胞尺寸为a = 46.66 A，b = 47.61 A和c = 54.05 A的空间群I222中结晶，并采用A形构型。所有的RNA和DNA糖均呈C3'-endo构型，反构型的糖基角以及除两个反角以外的大部分g +的C4'-C5'扭转角，与C3'-endo刚性核苷酸假设相符。腺嘌呤凸起被环出，并且也处于抗C3'-内构象。该凸起涉及与对称相关分子的小沟中的末端三对碱基（C9.g10）的碱基三重（C.g）*相互作用。 g15的2'羟基与g17的O2P和O5'氢键连接，跳过了凸起的腺嘌呤a16并稳定了杂种的糖磷酸骨架。凸出的腺嘌呤位点的氢键和骨架构象与蛋白质-RNA复合物的晶体结构中的非常相似。

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