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首页> 外文期刊>Journal of Molecular Biology >DETERMINATION OF SEQUENCE SPECIFICITY BETWEEN A PLASMID REPLICATION INITIATOR PROTEIN AND THE ORIGIN OF REPLICATION
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DETERMINATION OF SEQUENCE SPECIFICITY BETWEEN A PLASMID REPLICATION INITIATOR PROTEIN AND THE ORIGIN OF REPLICATION

机译：确定质粒复制启动子蛋白质和复制来源之间的序列特异性

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摘要

Staphylococcal plasmids of the pT181 family replicate by a rolling circle mechanism, requiring the activities of a plasmid-specified Rep protein. The initiation event involves site-specific phosphodiester bond cleavage by Rep within the replication origin, ori. In vitro the Rep proteins also display type-I topoisomerase activity specific for this plasmid family Although the single site of bond cleavage, ICR II, is conserved among all members of the pT181 family, the plasmid-specific Rep proteins are able to discriminate between family members in vivo, initiating replication only from the cognate origin. The basis of such specificity is believed to be due to a non-covalent binding interaction between Rep and a DNA sequence adjacent to the site of phosphodiester bond cleavage.
机译：pT181家族的葡萄球菌质粒通过滚环机制复制，需要质粒特异性Rep蛋白的活性。起始事件涉及复制起点ori中Rep的位点特异性磷酸二酯键裂解。在体外，Rep蛋白还表现出对该质粒家族特有的I型拓扑异构酶活性。尽管在pT181家族的所有成员中保守的单键切割位点ICR II，但质粒特异的Rep蛋白能够区分家族成员体内，仅从同源起源开始复制。据信这种特异性的基础是由于Rep和与磷酸二酯键切割位点相邻的DNA序列之间的非共价结合相互作用。

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