• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Molecular Biology >Evidence for DNA bending at the T7 RNA polymerase promoter.
【24h】

Evidence for DNA bending at the T7 RNA polymerase promoter.

机译：T7 RNA聚合酶启动子发生DNA弯曲的证据。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Phage T7 RNA polymerase is the only DNA-dependent RNA polymerase for which we have a high-resolution structure of the promoter-bound complex. Recent studies with the more complex RNA polymerases have suggested a role for DNA wrapping in the initiation of transcription. Here, circular permutation gel retardation assays provide evidence that the polymerase does indeed bend its promoter DNA. A complementary set of experiments employing differential phasing from an array of phased A-tracts provides further evidence for both intrinsic and polymerase-induced bends in the T7 RNA polymerase promoter DNA. The bend in the complex is predicted to be about 40-60 degrees and to be centered around positions -2 to +1, at the start site for transcription, while the intrinsic bend is much smaller (about 10 degrees ). These results, viewed in the light of a recent crystal structure for the complex, suggest a mechanism by which binding leads directly to bending. Bending at the start site would then facilitate the melting necessary to initiate transcription. Copyright 2000 Academic Press.
机译：噬菌体T7 RNA聚合酶是唯一的DNA依赖的RNA聚合酶，对于它，我们具有与启动子结合的复合物的高分辨率结构。最近对更复杂的RNA聚合酶的研究表明，DNA包裹在转录起始过程中发挥了作用。在此，圆形置换凝胶阻滞测定法提供了证据，证明聚合酶确实弯曲了其启动子DNA。一组互补的实验采用了一系列相控A道的差分相位，为T7 RNA聚合酶启动子DNA的固有弯曲和聚合酶诱导的弯曲提供了进一步的证据。预测复合物中的弯曲大约为40-60度，并在转录的起始位点以-2至+1位置为中心，而固有弯曲要小得多（大约10度）。从该配合物的最新晶体结构来看，这些结果表明了结合直接导致弯曲的机理。然后在起始位点弯曲将促进启动转录所必需的融解。版权所有2000学术出版社。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号