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首页> 外文期刊>Journal of Molecular Biology >The 1.5 A crystal structure of a bleomycin resistance determinant from bleomycin-producing Streptomyces verticillus.
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The 1.5 A crystal structure of a bleomycin resistance determinant from bleomycin-producing Streptomyces verticillus.

机译：博来霉素生产性链霉菌的博来霉素抗性决定簇的1.5 A晶体结构。

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摘要

Bleomycin (Bm)-binding protein, designated BLMA, which is a Bm resistance determinant from Bm-producing Streptomyces verticillus, was crystallized in a form suitable for X-ray diffraction analysis. The diffraction intensity data were collected up to a resolution of 1.5 A with a merging R-value of 0.054 at a completeness of 94 %. The BLMA structure, determined by the single isomorphous replacement method including the anomalous scattering effect (SIR-AS) at a resolution of 2.0 A, was refined at 1.5 A resolution. The final R-factor was 19.0 % and R(free) was 22.1 % including 91 water molecules. The crystal packing showed a dimer form, which was generated by arm exchange. The 1.5 A high-resolution experiment allowed an analysis of the side-chain disorder of BLMA. The structural comparison of BLMA with a homologous protein from Streptoalloteichus hindustanus, designated Shble protein, showed that a Ser100-Gly103 loop was farther from the groove, which is a Bm-binding site, in BLMA than in the Shble protein. Furthermore the hydrophobicity of the groove in BLMA is much lower than that in the Shble protein. The structural differences between these proteins may be responsible for the observation that a half-saturating concentration (K(1/2)) of Bm is higher for BLMA than for the Shble protein. Copyright 2000 Academic Press.
机译：博莱霉素（Bm）结合蛋白（称为BLMA）是适合于生产Bm的链霉菌（Verticillus）的Bm抗性决定子，以适合X射线衍射分析的形式结晶。收集的衍射强度数据分辨率高达1.5 A，合并R值为0.054，完整性为94％。通过单一同构置换方法确定的BLMA结构（包括分辨率为2.0 A的异常散射效应（SIR-AS））以1.5 A的分辨率精制。最终的R因子为19.0％，R（游离）为22.1％，包括91个水分子。晶体堆积物显示二聚体形式，其通过臂交换而产生。 1.5的高分辨率实验可以分析BLMA的侧链疾病。 BLMA与来自印度斯坦链球菌的同源蛋白（称为Shble蛋白）的结构比较显示，Ser100-Gly103环比BLMA中的距Bm结合位点的沟更远。此外，BLMA中凹槽的疏水性远低于Shble蛋白中的凹槽。这些蛋白质之间的结构差异可能是观察到的结果：BLMA的Bm的半饱和浓度（K（1/2））比Shble蛋白质高。版权所有2000学术出版社。

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