• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Molecular Biology >Differential nucleosome positioning on Xenopus oocyte and somatic 5 S RNA genes determines both TFIIIA and H1 binding: A mechanism for selective H1 repression
【24h】

Differential nucleosome positioning on Xenopus oocyte and somatic 5 S RNA genes determines both TFIIIA and H1 binding: A mechanism for selective H1 repression

机译：在非洲爪蟾卵母细胞和体细胞5 S RNA基因上的差异核小体定位决定TFIIIA和H1结合：选择性抑制H1的机制

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In Xenopus somatic cells histone H1 effects the transcriptional repression of oocyte type 5 S RNA genes, without altering the transcription of the somatic type 5 S RNA genes. Using an unambiguous nucleosome mapping method we find substantial differences between the multiple in vitro nucleosome positions on the two types of genes. These nucleosome positions determine both transcription factor and H1 binding, allowing TFIIIA to bind more efficiently to nucleosomes containing the somatic 5 S RNA gene than to nucleosomes on the oocyte 5 S RNA gene. Significantly, in a binding competition between TFIIIA and H1, TFIIIA preferentially binds to the somatic nucleosome whereas H1 preferentially binds to the oocyte nucleosome, excluding TFIIIA binding. These results strongly suggest that nucleosome positioning plays a key role in the regulation of transcription of 5 S RNA genes and provide a molecular mechanism for the selective repression of the oocyte 5 S RNA genes by H1. (C) 1998 Academic Press. [References: 60]
机译：在非洲爪蟾的体细胞中，组蛋白H1会影响卵母细胞5 S RNA基因的转录抑制，而不会改变5 S RNA基因的转录。使用明确的核小体作图方法，我们发现两种类型基因的多个体外核小体位置之间存在实质性差异。这些核小体的位置决定了转录因子和H1的结合，从而使TFIIIA与包含体细胞5 S RNA基因的核小体比与卵母细胞5 S RNA基因上的核小体更有效地结合。明显地，在TFIIIA和H1之间的结合竞争中，TFIIIA优先结合体细胞核小体，而H1优先结合卵母细胞核小体，但不包括TFIIIA结合。这些结果强烈提示核小体定位在5 S RNA基因转录的调控中起关键作用，并为H1选择性抑制卵母细胞5 S RNA基因提供了分子机制。 （C）1998年学术出版社。 [参考：60]

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号