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首页> 外文期刊>Journal of Molecular Biology >Time-resolved detection of transient movement of helix F in spin-labelled pharaonis sensory rhodopsin II.
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Time-resolved detection of transient movement of helix F in spin-labelled pharaonis sensory rhodopsin II.

机译：时间分辨检测自旋标记的法老鸟感觉视紫红质II中螺旋F的瞬时运动。

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摘要

Sensory rhodopsin II (also called phoborhodopsin) from the archaeal Natronobacterium pharaonis (pSRII) functions as a repellent phototaxis receptor. The excitation of the receptor by light triggers the activation of a transducer molecule (pHtrII) which has close resemblance to the cytoplasmic domain of bacterial chemotaxis receptors. In order to elucidate the first step of the signal transduction chain, the accessibility as well as static and transient mobility of cytoplasmic residues in helices F and G were analysed by electron paramagnetic resonance spectroscopy. The results indicate an outward tilting of helix F during the early steps of the photocycle which is sustained until the reformation of the initial ground state. Co-expression of pSRII with a truncated fragment of pHtrII affects the accessibility and/or the mobility of certain spin-labelled residues on helices F and G. The results suggest that these sites are located within the binding surface of the photoreceptor with its transducer. Copyright 2000 Academic Press.
机译：来自古细菌嗜盐杆菌（pSRII）的感觉视紫红质II（也称为磷酸视紫红质）充当驱光趋光性受体。光对受体的激发触发了换能器分子（pHtrII）的激活，该分子与细菌趋化性受体的胞质域非常相似。为了阐明信号转导链的第一步，通过电子顺磁共振波谱分析了螺旋F和G中胞质残基的可及性以及静态和瞬时迁移性。结果表明，在光循环的早期阶段，螺旋线F向外倾斜，一直持续到初始基态重新形成为止。 pSRII与pHtrII的截短片段的共表达会影响螺旋F和G上某些自旋标记的残基的可及性和/或迁移率。结果表明，这些位点位于感光体与其传感器之间的结合表面内。版权所有2000学术出版社。

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