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首页> 外文期刊>Journal of Molecular Biology >Endoribonuclease RegB from bacteriophage T4 is necessary for the degradation of early but not middle or late mRNAs.
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Endoribonuclease RegB from bacteriophage T4 is necessary for the degradation of early but not middle or late mRNAs.

机译：噬菌体T4的核糖核酸内切酶RegB对于降解早期但不是中晚期的mRNA是必需的。

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摘要

The RegB endoribonuclease from bacteriophage T4 cleaves early mRNAs specifically in the middle of the sequence GGAG. We show here that RegB is required for the degradation of bulk T4 early mRNA. In the absence of RegB, the chemical half-life of early transcripts is increased nearly fourfold, whereas their functional half-life is increased twofold. RegB also regulates the translation of several prereplicative genes. The synthesis of several early proteins is down-regulated, probably as a consequence of RegB cleavages in the Shine-Dalgarno sequence of these genes. The synthesis of several other proteins is up-regulated, suggesting that processing by RegB might improve translation by changing the conformation of a transcript. In contrast, RegB does not affect the average half-life of middle and late mRNA. An analysis of the susceptibility to RegB of many GGAG motifs carried by these mRNA species showed that most middle and all late GGAG-carrying mRNAs escape RegB processing in spite of the fact that the enzyme is acting at least until ten minutes post-infection. The sensitivity or resistance to RegB observed during phage infection could be reproduced in uninfected Escherichia coli cells and in vitro. This shows that the GGAG-carrying RNAs that are uncut during T4 infection are not substrates, whatever the period of the T4 cycle when the transcripts are made. Copyright 2000 Academic Press.
机译：来自噬菌体T4的RegB核糖核酸内切酶特异性地在序列GGAG的中间切割早期的mRNA。我们在这里显示RegB是降解T4早期mRNA所需的。在没有RegB的情况下，早期转录本的化学半衰期增加了近四倍，而它们的功能半衰期却增加了两倍。 RegB还调节几个复制前基因的翻译。几种早期蛋白质的合成被下调，可能是由于这些基因的Shine-Dalgarno序列中的RegB裂解所致。其他几种蛋白质的合成也被上调，这表明RegB的加工可能会通过改变转录本的构象来改善翻译。相反，RegB不会影响中晚期mRNA的平均半衰期。对由这些mRNA物种携带的许多GGAG基序对RegB的敏感性的分析表明，尽管该酶至少在感染后十分钟内起作用，但大多数中等和所有晚期GGAG携带的mRNA都逃避了RegB的加工。在噬菌体感染期间观察到的对RegB的敏感性或抗性可以在未感染的大肠杆菌细胞和体外复制。这表明在T4感染过程中未切割的GGAG携带RNA不是底物，无论转录本在T4循环的周期如何。版权所有2000学术出版社。

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