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首页> 外文期刊>Journal of Molecular Biology >Mimicry of human IgE epitopes by anti-idiotypic antibodies.
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Mimicry of human IgE epitopes by anti-idiotypic antibodies.

机译：抗独特型抗体模仿人IgE表位。

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According to Jerne's network hypothesis, the binding site of an anti-idiotypic antibody also represents the internal image of an epitope present on a foreign, or even a self antigen. In recent years, antigen mimicry has been defined at the molecular level for some xeno-antigens. However, until now there has been no demonstration of structural mimicry between a human anti-idiotypic antibody and a self structure. To address this question, we used human IgE as the self structure and a well-defined anti-human IgE mAb (BSW17). We describe the isolation of two anti- idiotypic antibodies specific for the anti-IgE antibody BSW17 from a non-immune human Fab phage display library. Interestingly, these two anti-idiotypic antibodies mimic the same molecular surface region as a previously described IgE peptide mimotope isolated by panning on BSW17, but they cover a much larger epitope on the IgE molecule. Accordingly, immunisation of rabbits with the two anti-idiotypic antibodies induced high-affinity antibodies with the same characteristics as BSW17. Thus, our data demonstrate that it is possible to isolate anti-idiotypic antibodies derived from the human genome without the need for hyperimmunization, and confirm Jerne's hypothesis that both foreign antigens and self structures can be mimicked by our own immunoglobulins. Copyright 2000 Academic Press.
机译：根据耶恩（Jerne）网络假说，抗独特型抗体的结合位点也代表存在于外源甚至自身抗原上的表位的内部图像。近年来，在某些异种抗原的分子水平上已经定义了抗原模拟。但是，到目前为止，还没有人抗独特型抗体和自身结构之间结构模仿的证明。为了解决这个问题，我们使用人IgE作为自身结构，并使用定义明确的抗人IgE mAb（BSW17）。我们描述了从非免疫人Fab噬菌体展示文库中分离出两种针对抗IgE抗体BSW17的抗独特型抗体。有趣的是，这两种抗独特型抗体模拟与通过在BSW17上淘选分离的先前描述的IgE肽模拟表位相同的分子表面区域，但是它们覆盖了IgE分子上更大的表位。因此，用两种抗独特型抗体免疫兔子会诱导出具有与BSW17相同特征的高亲和力抗体。因此，我们的数据表明，无需超免疫就可以分离源自人类基因组的抗独特型抗体，并证实了耶恩的假说，即我们自己的免疫球蛋白可以模仿外源抗原和自身结构。版权所有2000学术出版社。

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