Jack bean urease (EC 3.5.1.5) aggregation monitored by dynamic and static light scattering

Follmer C; Pereira FV; da Silveira NP; Carlini CR

首页> 外文期刊>Biophysical Chemistry: An International Journal Devoted to the Physical Chemistry of Biological Phenomena >Jack bean urease (EC 3.5.1.5) aggregation monitored by dynamic and static light scattering

【24h】

Jack bean urease (EC 3.5.1.5) aggregation monitored by dynamic and static light scattering

机译：通过动态和静态光散射监控波豆脲酶（EC 3.5.1.5）聚集

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

Aggregation of jack bean urease (JBU) is involved in many alterations of its biological properties, notably the ureolytic and entomotoxic activities. In order to investigate this phenomenon, protein aggregates were characterized by dynamic (DLS) and static light scattering (SLS) spectroscopies through determination of apparent hydrodynamic radii, the average molecular masses, radii of gyration and second virial coefficients. No effect of disulfide reducing agents on protein association was observed contrasting with previous reports implicating their function in the prevention of JBU aggregation. The influence of freeze-thawing cycles on protein aggregation was also investigated. Our results showed that after freeze-thawing cycles the native form of JBU with apparent hydrodynamic radius of 7 nm and radius of gyration of 12 nm is replaced by high-order oligomers and this aggregation is not reverted neither by dithiothreitol (DTT) treatment nor by high concentration of salts. Altogether the data help to understand the complex behavior of JBU in solution and may correlate with the diversity of biological properties of this enzyme. (C) 2004 Elsevier B.V. All rights reserved.

机译：杰克豆脲酶（JBU）的聚集涉及其生物学特性的许多变化，特别是尿素分解和昆虫毒性活性。为了研究这种现象，通过测定表观流体动力学半径，平均分子量，回转半径和第二病毒系数，通过动态（DLS）和静态光散射（SLS）光谱对蛋白质聚集体进行表征。与以前的报道暗示其在预防JBU聚集中的功能相反，未观察到二硫化物还原剂对蛋白质缔合的影响。还研究了冻融循环对蛋白质聚集的影响。我们的结果显示，在冻融循环后，JBU的天然形式的表观流体动力学半径为7 nm，回转半径为12 nm，被高阶低聚物取代，并且这种聚集作用既不会被二硫苏糖醇（DTT）处理也不会被还原。高浓度的盐。总而言之，数据有助于了解JBU在溶液中的复杂行为，并且可能与该酶的生物学特性相关。（C）2004 Elsevier B.V.保留所有权利。

著录项

来源
《Biophysical Chemistry: An International Journal Devoted to the Physical Chemistry of Biological Phenomena》 |2004年第1期|共9页
作者
Follmer C; Pereira FV; da Silveira NP; Carlini CR;
展开▼
作者单位

展开▼
收录信息
原文格式 PDF
正文语种 eng
中图分类生物化学;
关键词
urease; oligomerization; freeze-thawing; hydrodynamic radius; radius of gyration; CORRELATION SPECTROSCOPY; EC 3.5.1.5; POLYDISPERSITY; PROTEIN; CANATOXIN; ENZYME; TOXIN; FORM;

机译：脲酶;寡聚;冻融;流体力学半径;回转半径;相关光谱;EC 3.5.1.5;聚酯;蛋白质;卡那毒素;酶;毒素;形态;

相似文献

外文文献
中文文献
专利

1. Jack bean urease (EC 3.5.1.5) aggregation monitored by dynamic and static light scattering [J] . Follmer C, Pereira FV, da Silveira NP, Biophysical Chemistry: An International Journal Devoted to the Physical Chemistry of Biological Phenomena . 2004,第1期

机译：通过动态和静态光散射监控波豆脲酶（EC 3.5.1.5）聚集
2. Effect of chemical modification of histidines on the copper-induced oligomerization of jack bean urease (EC 3.5.1.5) [J] . Follmer C, Carlini UR Archives of Biochemistry and Biophysics . 2005,第1期

机译：组氨酸的化学修饰对铜诱导的菜豆脲酶低聚的影响（EC 3.5.1.5）
3. Absolute Aggregation Rate Constants in Aggregation of Kaolinite Measured by Simultaneous Static and Dynamic Light Scattering [J] . Marta Berka, James A.Rice Langmuir: The ACS Journal of Surfaces and Colloids . 2004,第15期

机译：通过同时进行静态和动态光散射测量的高岭石聚集体中的绝对聚集速率常数
4. Dynamics of light scattering matrix in a process of scatterers aggregation [C] . Sergey V. Romanov, Institute of Radio Engineering, Electronics, Nonlinear Dynamics and Structures in Biology and Medicine: Optical and Laser Technologies: International Workshop . 1997

机译：散射体聚集过程中光散射矩阵的动力学
5. Different particle size information obtained from static and dynamic laser light scattering [D] . Sun, Yong 2005

机译：从静态和动态激光散射获得的不同粒度信息
6. Canatoxin a toxic protein from jack beans (Canavalia ensiformis) is a variant form of urease (EC 3.5.1.5): biological effects of urease independent of its ureolytic activity. [O] . C Follmer, G B Barcellos, R B Zingali, 2001

机译：Canatoxin是一种来自波豆（Canavalia ensiformis）的有毒蛋白质是脲酶的一种变体形式（EC 3.5.1.5）：脲酶的生物学效应与其尿素分解活性无关。
7. Canatoxin, a toxic protein from jack beans (Canavalia ensiformis), is a variant form of urease (EC 3.5.1.5): biological effects of urease independent of its ureolytic activity. [O] . Follmer, C, Barcellos, G B, Zingali, R B, 2001

机译：Canatoxin是一种来自波豆（Canavalia ensiformis）的有毒蛋白质，是脲酶的一种变体形式（EC 3.5.1.5）：脲酶的生物学效应与其尿素分解活性无关。

Jack bean urease (EC 3.5.1.5) aggregation monitored by dynamic and static light scattering

摘要

著录项

相似文献

相关主题

期刊订阅