Development of reversed passive latex agglutination for detection of Thai cobra (Naja kaouthia) venom.

Khow O; Wongtongkam N; Pakmanee N; Omori Satoh-T; Sitprija V

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Development of reversed passive latex agglutination for detection of Thai cobra (Naja kaouthia) venom.

机译：反向被动胶乳凝集技术的发展，用于检测泰国眼镜蛇（Naja kaouthia）毒液。

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A simple, rapid, and sensitive diagnostic kit for detecting Thai cobra (Naja kaouthia) venom was developed using latex particles sensitized with venom specific immunoglobulin. The kit is capable of detecting 25-50 ng/ml of Thai cobra venom. The capability was not affected by human plasma. Specificity of the kit was proven using snake venoms from Vipera russelli, Calloselasma rhodostoma, Trimeresurus albolabris, Naja siamensis, Ophiophagus hannah, and Bungarus fasciatus. The diagnostic kit does not lose its capability under refrigeration for two months and by lyophilization.

机译：开发了一种简单，快速，灵敏的诊断试剂盒，用于检测用泰国毒蛇特异性免疫球蛋白敏化的乳胶颗粒来检测泰国眼镜蛇（眼镜蛇）毒液。该试剂盒能够检测25-50 ng / ml的泰国眼镜蛇毒液。该能力不受人体血浆的影响。该试剂盒的特异性通过蛇毒，Vi蛇，红假单胞菌，Trimeresurus albolabris，Naja siamensis，Ophiophagus hannah和Bungarus fasciatus的蛇毒来证明。诊断试剂盒在冷藏两个月和冻干后不会失去其功能。

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来源
《Journal of natural toxins》 |1999年第2期|共8页
作者
Khow O; Wongtongkam N; Pakmanee N; Omori Satoh-T; Sitprija V;
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正文语种 eng
中图分类毒物学（毒理学）;
关键词
Cobra Venoms; Immunoglobulins; Latex Fixation Tests; Snake Venoms; IgG; 眼镜蛇毒液类; 免疫球蛋白类; 胶乳结合试验; 蛇毒液类;

机译：Cobra Venoms;Immunoglobulins;Latex Fixation Tests;Snake Venoms;IgG;眼镜蛇毒液类;免疫球蛋白类;胶乳结合试验;蛇毒液类;

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1. Development of reversed passive latex agglutination for detection of Thai cobra (Naja kaouthia) venom. [J] . Khow O, Wongtongkam N, Pakmanee N, Journal of natural toxins . 1999,第2期

机译：反向被动胶乳凝集技术的发展，用于检测泰国眼镜蛇（Naja kaouthia）毒液。
2. Development of the Reverse Passive Latex Agglutination Method for the Detection and Quantification of the Genus Nitrospira in the Wastewater Treatment Process [J] . Okumura Takekazu, Nagai Fumiko, Yamamoto Shuta Bioscience, Biotechnology, and Biochemistry . 2008,第2期

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5. Demography of the monocled cobra (Naja kaouthia) in the central region of Thailand [D] . Chaitae, Apinya 2011

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Development of reversed passive latex agglutination for detection of Thai cobra (Naja kaouthia) venom.

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