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首页> 外文期刊>Journal of natural toxins >Identification of tetrodotoxin in marine gastropods implicated in food poisoning.
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Identification of tetrodotoxin in marine gastropods implicated in food poisoning.

机译：鉴定与食物中毒有关的海洋腹足动物中河豚毒素。

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摘要

Attempts were made to identify the toxin in the gastropod Zeuxis samiplicutus that was responsible for the recent food poisoning incidents in southern Zhejiang, Mainland China. Symptoms associated with the poisonings included paralysis, coma, vomiting, and aphasia. The remaining specimens of gastropod were assayed for tetrodotoxin toxicity (TTX). The range of specimen toxicity was found to be 4 - 186 mouse units (MU), and the average toxicity was 111 +/- 45 MU. The toxin was partially purified from the methanol extract of the gastropod by ultrafitration and Bio-Gel P-2 column chromatography. Cellulose acetate membrane electrophoresis, TLC, and HPLC analyses demonstrated that the toxin contained TTX. It was concluded that the causative agent of the above food poisoning was TTX.
机译：试图鉴定腹足动物Zeuxis samiplicutus中的毒素，该毒素是最近在中国大陆浙江南部发生的食物中毒事件的起因。与中毒有关的症状包括麻痹，昏迷，呕吐和失语。测定了腹足动物的其余标本的河豚毒素毒性（TTX）。发现标本毒性范围为4-186小鼠单位（MU），平均毒性为111 +/- 45 MU。通过超滤和Bio-Gel P-2柱色谱法从腹足动物的甲醇提取物中部分纯化了毒素。醋酸纤维素膜电泳，TLC和HPLC分析表明该毒素含有TTX。结论是上述食物中毒的病原是TTX。

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