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Determination of artemisinin and moisture content of Artemisia annua L. dry powder using a hand-held near infrared spectroscopy device

机译:使用手持式近红外光谱仪测定青蒿素和青蒿干粉中的水分含量

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摘要

A rapid, low-cost method, based on near-infrared spectroscopy (NIR), was developed to determine artemisinin and moisture content in dry powder of Artemisia annua (A. annua) leaves. A calibration set of 60 samples and validation set of 40 samples of A. annua hybrids exhibiting artemisinin content of between 0.7% and 1.6% was used. Results of partial least squares modelling indicated that NIR was accurate in predicting artemisinin content. Root mean square error values of cross-validation (RMSECV) and prediction (RMSEV) of 0.1% were calculated, in both cases. A model of moisture content was particularly accurate with RMSECV and RMSEP values of 0.8% (R=0.99) and 1.4%, respectively.
机译:开发了一种基于近红外光谱(NIR)的快速,低成本方法,用于测定青蒿(A. annua)叶片干粉中的青蒿素和水分含量。使用60个样品的校准集和40个样品的验证集,其青蒿素含量在0.7%至1.6%之间。偏最小二乘建模的结果表明,NIR可以准确预测青蒿素的含量。在这两种情况下,交叉验证的均方根误差值(RMSECV)和预测值(RMSEV)均计算为0.1%。水分含量模型特别准确,RMSECV和RMSEP值分别为0.8%(R = 0.99)和1.4%。

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