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首页> 外文期刊>Journal of Non-Crystalline Solids: A Journal Devoted to Oxide, Halide, Chalcogenide and Metallic Glasses, Amorphous Semiconductors, Non-Crystalline Films, Glass-Ceramics and Glassy Composites >Scaffolds with encapsulated DNA for non-viral gene delivery
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Scaffolds with encapsulated DNA for non-viral gene delivery

机译：带有DNA封装的支架，用于非病毒基因递送

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摘要

A novel approach to create a biodegradable scaffold containing a condensed DNA core and a biodegradable polymer shell has been demonstrated. In this approach, the extended DNA chains were first collapsed into compact globules in a poor solvent, containing 94% DMF + 6%TE buffer, where the shrinkage in the radius of gyration was by a factor of about 9. The condensed DNA globule was then encapsulated by using a tri-block copolymer of polylactide-polyethylene glycol)-polylactide (LEL). The encapsulated DNA protected it from degradation during the electro-spinning process, which also removed the undesirable DMF solvent, preserving the DNA in the dry nanofibrous scaffold. An enhanced transfection activity was observed when the scaffold was used to deliver the embedded DNA to cells adhered to the scaffold. (c) 2006 Elsevier B.V. All rights reserved.
机译：已经证明了一种新颖的方法来制备包含缩合的DNA核和可生物降解的聚合物壳的可生物降解的支架。在这种方法中，首先将延伸的DNA链在不良溶剂（包含94％DMF + 6％TE缓冲液）中折叠成紧密的小球，其中回转半径的收缩约为9倍。然后通过使用聚丙交酯-聚乙二醇-聚丙交酯（LEL）的三嵌段共聚物进行封装。包封的DNA可防止其在电纺丝过程中降解，这也去除了不良的DMF溶剂，从而将DNA保留在干燥的纳米纤维支架中。当使用支架将包埋的DNA递送至粘附于支架的细胞时，观察到增强的转染活性。 （c）2006 Elsevier B.V.保留所有权利。

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