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首页> 外文期刊>Journal of New Seeds >Varietal Identification in Paddy (Oryza sativa L.) and Moong (Phaseolus mungo L.) by Gel Electrophoresis of Soluble Seed Proteins
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Varietal Identification in Paddy (Oryza sativa L.) and Moong (Phaseolus mungo L.) by Gel Electrophoresis of Soluble Seed Proteins

机译：可溶性种子蛋白的凝胶电泳鉴定稻（Oryza sativa L.）和蒙（Phaseolus mungo L.）的品种

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摘要

Varietal development and its identification is one of the most important aspects of seed industry and seed trade. With the increase in the number of varieties in each crop, it has become difficult to identify and characterize these varieties on the basis of morphological characters alone. It has led to the exploration of new stable characters including genetic makeup to be used as markers for varietal identification. The present study includes the identification of different varieties (10) of paddy and (6) of moong on the basis of their protein profile. Protein was extracted from dry seeds using Laemmli buffer. The extracted protein samples were run at 150-200 volts for 5 hours using Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis (SDS-PAGE). After fixation and silver staining varieties were identified according to their differences in the banding pattern and staining intensities. It was observed that among paddy varieties 2 Basmati varieties were showing a clear distinction from the rest of varieties by the presence of a characteristics low molecular weight protein band at position 19. One characteristic band no. 16 was present only in PR-111 paddy variety. Presence of a band no. 20 and absence of a band no. 8 in electiophoretic pattern of moong varieties SML (local) and PS-105, respectively, could be used for distinction of these varieties from others.
机译：品种开发及其鉴定是种子产业和种子贸易的最重要方面之一。随着每种作物品种数量的增加，仅基于形态特征就难以鉴定和表征这些品种。它导致了对新的稳定特征的探索，包括遗传构成，可用作基因鉴定的标记。本研究包括根据其蛋白质谱鉴定稻的不同品种（10）和红花（6）。使用Laemmli缓冲液从干燥种子中提取蛋白质。使用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE），将提取的蛋白质样品在150-200伏特下运行5小时。固色和银染后，根据条带图样和染色强度的差异，鉴定出不同的染色品种。观察到，在水稻品种中，有2个巴斯马蒂品种与其他品种相比有明显区别，这是由于在位置19处存在特征性低分子量蛋白带。仅PR-111水稻品种中有16种。乐队编号20，没有乐队编号。 Moong品种SML（本地）和PS-105的电泳图谱可分别用于区分这些品种。

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