...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of neuroendocrinology >Functional Development of the Zebrafish Pineal Gland: Light-Induced Expression of Period2 is Required for Onset of the Circadian Clock.
【24h】

Functional Development of the Zebrafish Pineal Gland: Light-Induced Expression of Period2 is Required for Onset of the Circadian Clock.

机译：斑马鱼松果体的功能发育：昼夜节律的发生需要光诱导的Period2表达。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Abstract In zebrafish, the pineal gland is a photoreceptive organ that contains an intrinsic circadian oscillator and exhibits rhythmic arylalkylamine-N-acetyltransferase (zfaanat2) mRNA expression. In the present study, we investigated the role of light and of a clock gene, zperiod2 (zper2), in the development of this rhythm. Analysis of zfaanat2 mRNA expression in the pineal gland of 3-day-old zebrafish embryos after exposure to different photoperiodic regimes indicated that light is required for proper development of the circadian clock-controlled rhythmic expression of zfaanat2, and that a 1-h light pulse is sufficient to initiate this rhythm. Analysis of zper2 mRNA expression in zebrafish embryos exposed to different photoperiodic regimes indicated that zper2 expression is transiently up-regulated by light but is not regulated by the circadian oscillator. To establish the association between light-induced zper2 expression and light-induced clock-controlled zfaanat2 rhythm, zPer2 knock-down experiments were performed. The zfaanat2 mRNA rhythm, induced by a 1-h light pulse, was abolished in zPer2 knock-down embryos. These experiments indicated that light-induced zper2 expression is crucial for establishment of the clock-controlled zfaanat2 rhythm in the zebrafish pineal gland.
机译：摘要在斑马鱼中，松果体是一个感光器官，它包含一个内在的昼夜节律振荡器，并表现出有节奏的芳基烷基胺-N-乙酰基转移酶（zfaanat2）mRNA表达。在本研究中，我们调查了光和时钟基因zperiod2（zper2）在此节律发展中的作用。对3天大的斑马鱼胚胎在不同光周期条件下暴露的松果体中zfaanat2 mRNA表达的分析表明，光是zfaanat2的昼夜节律控制的节律性表达正常发育所必需的，并且有1小时的光脉冲足以启动这种节奏。暴露于不同光周期状态的斑马鱼胚胎中zper2 mRNA表达的分析表明，zper2表达受光短暂上调，但不受昼夜节律调节。为了建立光诱导的zper2表达与光诱导的时钟控制的zfaanat2节奏之间的关联，进行了zPer2敲低实验。 1 h光脉冲诱导的zfaanat2 mRNA节律在zPer2敲除胚胎中被废除。这些实验表明，光诱导的zper2表达对于在斑马鱼松果体中建立时钟控制的zfaanat2节奏至关重要。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号