...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of neuroendocrinology >Expression of functional ciliary neurotrophic factor receptors in immortalized gonadotrophin-releasing hormone-secreting neurones.
【24h】

Expression of functional ciliary neurotrophic factor receptors in immortalized gonadotrophin-releasing hormone-secreting neurones.

机译：功能性睫状神经营养因子受体在永生性促性腺激素释放激素分泌神经元中的表达。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Abstract Ciliary neurotrophic factor (CNTF), a cytokine of the interleukin-6 superfamily, is known to exert pleiotropic actions, including regulation of food intake and permissive effects on reproduction, by facilitating the release of gonadotrophin-releasing hormone (GnRH) and gonadotrophins. CNTF activates membrane receptors (CNTF-Rs) composed of one ligand-specific binding subunit, defined CNTFRalpha, and two signal transducing subunits, termed leukaemia inhibitory factor receptor (LIFR) and gp130. However, it is not clear whether the effects of CNTF on GnRH release result from either a direct or an indirect action on GnRH-secreting hypothalamic neurones, or from a combination of these events. The hypothesis of a direct effect of CNTF was thus tested using the GT1-7 GnRH-secreting cell line. CNTF-R expression and CNTF-induced modulation of the Janus kinase (JAK2)-signal transducer and activator of transcription 3 (STAT3) pathway and of GnRH release were evaluated. GT1-7 cells were found to express CNTFRalpha, LIFR and gp130 genes, as shown by reverse transcription-polymerase chain reaction analysis, and the corresponding proteins, analysed by immunofluorescence and western blot. CNTFRalpha, LIFR and gp130 immunoreactive bands had an approximate size of 50, 190 and 130 kDa, respectively. Treatment of GT1-7 cells with 10(-12) M CNTF for 15-60 min resulted in a marked and transient increase of STAT3 phosphorylation via activation of JAK2. A 30-min exposure of GT1-7 cells to different CNTF concentrations increased the accumulation of GnRH into the culture medium, with a maximal effect at 10(-11) M. In conclusion, the present results provide new information about the regulation of the reproductive axis by CNTF, and suggest that it might operate at the hypothalamic level by directly influencing the activity of GnRH-secreting neurones, in addition to the possible indirect effects via interneurones proposed by previous studies.
机译：摘要睫状神经营养因子（CNTF）是白介素6超家族的一种细胞因子，它通过促进促性腺激素释放激素（GnRH）和促性腺激素的释放而发挥多效性作用，包括调节食物摄入量和对生殖的许可作用。 CNTF激活由一个配体特异性结合亚基（定义为CNTFRalpha）和两个信号转导亚基（称为白血病抑制因子受体（LIFR）和gp130）组成的膜受体（CNTF-Rs）。然而，尚不清楚CNTF对GnRH释放的影响是由于对分泌GnRH的下丘脑神经元的直接作用还是间接作用，还是这些事件的组合。因此，使用分泌GT1-7 GnRH的细胞系测试了CNTF直接作用的假设。评估了CNTF-R的表达以及CNTF诱导的Janus激酶（JAK2）-信号转导子和转录激活因子3（STAT3）通路以及GnRH释放的调节。如逆转录-聚合酶链反应分析所示，GT1-7细胞表达CNTFRalpha，LIFR和gp130基因，而相应的蛋白质则通过免疫荧光和蛋白质印迹分析。 CNTFRalpha，LIFR和gp130免疫反应带的大小分别约为50、190和130 kDa。用10（-12）M CNTF处理GT1-7细胞15-60分钟，通过激活JAK2导致STAT3磷酸化的显着和瞬时增加。 GT1-7细胞在不同CNTF浓度下暴露30分钟会增加GnRH在培养基中的积累，在10（-11）M时作用最大。总而言之，本结果提供了有关GnRH调控的新信息。 CNTF认为它可能通过直接影响GnRH分泌神经元的活性而在下丘脑水平起作用，此外还可能通过先前的研究提出通过中间神经元的间接作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号