...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of neuroendocrinology >Characterization of gonadotrophin-releasing hormone precursor cDNA in the old world mole-rat cryptomys hottentotus pretoriae: high degree of identity with the new world Guinea pig sequence.
【24h】

Characterization of gonadotrophin-releasing hormone precursor cDNA in the old world mole-rat cryptomys hottentotus pretoriae: high degree of identity with the new world Guinea pig sequence.

机译：在旧世界的-鼠隐球菌hottentotus pretoriae中释放促性腺激素的激素前体cDNA的特征：与新世界豚鼠序列的高度同一性。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Abstract Regulation of pituitary gonadotrophins by the decapeptide gonadotrophin-releasing hormone 1 (GnRH1) is crucial for the development and maintenance of reproductive functions. A common amino acid sequence for this decapeptide, designated as 'mammalian' GnRH, has been identified in all mammals thus far investigated with the exception of the guinea pig, in which there are two amino acid substitutions. Among hystricognath rodents, the members of the family Bathyergidae regulate reproduction in response to diverse cues. Thus, highveld mole-rats (Cryptomys hottentotus pretoriae) are social bathyergids in which breeding is restricted to a particular season in the dominant female, but continuously suppressed in subordinate colony members. Elucidation of reproductive control in these animals will be facilitated by characterization of their GnRH1 gene. A partial sequence of GnRH1 precursor cDNA was isolated and characterized. Comparative analysis revealed the highest degree of identity (86%) to guinea pig GnRH1 precursor mRNA. Nevertheless, the deduced amino acid sequence of the mole-rat decapeptide is identical to the 'mammalian' sequence rather than that of guinea pigs. Successful detection of GnRH1-synthesizing neurones using either a guinea pig GnRH1 riboprobe or an antibody against the 'mammalian' decapeptide is consistent with the guinea pig-like sequence for the precursor and the classic 'mammalian' form for the decapeptide. The high degree of identity in the GnRH1 precursor sequence between this Old World mole-rat and the New World guinea pig is consistent with the theory that caviomorphs and phiomorphs originated from a common ancestral line in the Palaeocene to mid Eocene, some 63-45 million years ago.
机译：摘要十肽促性腺激素释放激素1（GnRH1）对垂体促性腺激素的调节对于维持和维持生殖功能至关重要。迄今为止，除豚鼠（其中有两个氨基酸取代）外，在所有已研究的哺乳动物中均已鉴定出该十肽的通用氨基酸序列，称为“哺乳动物” GnRH。在Hystricognath啮齿动物中，Bathyergidae家族的成员根据各种线索调节繁殖。因此，高级mole鼠（Cryptomys hottentotus pretoriae）是社会霸王，其中优势雌性的繁殖被限制在特定的季节，但在下属殖民地成员中却被连续抑制。这些动物的GnRH1基因的特征将有助于阐明这些动物的生殖控制。分离并鉴定了GnRH1前体cDNA的部分序列。对比分析显示与豚鼠GnRH1前体mRNA的同一性最高（86％）。然而，推导的鼠鼠十肽的氨基酸序列与“哺乳动物”序列相同，而不是豚鼠。使用豚鼠GnRH1核糖探针或抗“哺乳动物”十肽的抗体成功检测GnRH1合成神经元，与前体的豚鼠样序列和十肽的经典“哺乳动物”形式相符。在这只旧世界的mole鼠和新世界的豚鼠之间，GnRH1前体序列的高度同一性与以下理论相吻合：caviomorph和phiomorph起源于古新世至始新世的共同祖先系，约63-45百万几年前。

著录项

相似文献

  • 外文文献
  • 中文文献
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号