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首页> 外文期刊>Journal of neurodegeneration & regeneration. >Bromodeoxyuridine labeling: Beware of the limitations and pitfalls
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Bromodeoxyuridine labeling: Beware of the limitations and pitfalls

机译：溴脱氧尿苷标签：谨防局限和陷阱

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摘要

Bromodeoxyuridine (BrdU) labeling is used for birthdat-ing and monitoring cell proliferation. It was originally developed to determine the proliferative index of tumors, and the chemical was used as an antiviral and antineoplastic agent. It was later introduced by Nowakowski et al. to determine the time of origin, migration, lineage, and fate of neuronal cells in the developing nervous system. BrdU labeling is the paradigm the most used to study and characterize, by immunohistochemistry and confocal microscopy, neurogenesis in the adult brain of mammals including in humans. In humans, newly generated neuronal cells, immunopositive for BrdU in the adult dentate gyrus (DG), were identified and characterized in tissue samples obtained postmortem from patients with cancer who had been administered with the drug as part of their therapy. BrdU is not a marker for cell proliferation; it is a marker of deoxyribonucleic acid (DNA) synthesis. Studying and quantifying neurogenesis with BrdU require distinguishing cell proliferation and neurogenesis from other events involving DNA synthesis.
机译：溴脱氧尿苷（BrdU）标记可用于出生和监测细胞增殖。它最初是为了确定肿瘤的增殖指数而开发的，该化学品被用作抗病毒和抗肿瘤药。后来由Nowakowski等人介绍。确定发育中的神经系统中神经元细胞的起源，迁移，沿袭和命运的时间。 BrdU标记是最常用于通过免疫组织化学和共聚焦显微镜研究和表征哺乳动物（包括人类）成年大脑中神经发生的范例。在人类中，从成年齿状回（DG）中BrdU免疫阳性的新生神经元细胞，在从接受过该药物治疗的癌症患者的死后组织样品中鉴定和鉴定。 BrdU不是细胞增殖的标志物。它是脱氧核糖核酸（DNA）合成的标志。用BrdU研究和量化神经发生需要将细胞增殖和神经发生与涉及DNA合成的其他事件区分开来。

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