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首页> 外文期刊>Biophysical Chemistry: An International Journal Devoted to the Physical Chemistry of Biological Phenomena >SIMULATION OF STATE 4 -] STATE 3 TRANSITION IN ISOLATED MITOCHONDRIA
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SIMULATION OF STATE 4 -] STATE 3 TRANSITION IN ISOLATED MITOCHONDRIA

机译：隔离线粒体中状态4-]状态3跃迁的模拟

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The mathematical dynamic model of oxidative phosphorylation developed previously and in the accompanying paper was modified to involve isolated mitochondria conditions; it was also used to simulate state 4 --> state 3 transition in rat liver mitochondria incubated with succinate as respiratory substrate and glucose-hexokinase as an ADP-regenerating system. Changes in the respiration rare, protonmotive force and reduction level of ubiquinone and cytochrome c as well as the internal (i) and external (e) ATP/ADP ratio between state 4 and slate 3 were calculated and compared with the experimental data. Flux control coefficients with respect to oxygen consumption flux for different reactions and processes of oxidative phosphorylation were simulated for different values of the respiration rate (state 4, state 3 and intermediate states). Flux control coefficients for the oxidation, phosphorylation and proton leak subsystems with respect to the oxidation, phosphorylation and proton leak fluxes for different values of the respiration rate were also calculated. These theoretical data were compared with the experimental results obtained in the frame of metabolic control analysis and the 'top-down' approach to this analysis. A good agreement was obtained. Simulated time courses of the respiration rate, the protonmotive force (Delta p) and other parameters after addition of a small amount of ADP to mitochondria in state 4 mimicked at least semiquantitatively the experimentally measured time courses of these parameters. It was concluded, therefore, that in the present stage, the model is able to reflect different properties of the oxidative phosphorylation system in a broad range of conditions fairly well, allows deeper insight into the mechanisms responsible for control and regulation of this process, and can be used for simulation of new experiments, thus inspiring experimental verification of the theoretical predictions. [References: 46]
机译：先前和随附论文中开发的氧化磷酸化的数学动力学模型进行了修改，以包含孤立的线粒体条件;它也被用来模拟大鼠肝线粒体中的状态4->状态3的转变，琥珀酸作为呼吸底物，葡萄糖己糖激酶作为ADP再生系统。计算了呼吸稀有性，质子动力和泛醌和细胞色素c的降低水平以及状态4和状态3之间的内部（i）和外部（e）ATP / ADP比的变化，并将其与实验数据进行了比较。针对呼吸速率的不同值（状态4，状态3和中间状态），针对不同反应和氧化磷酸化过程模拟了相对于耗氧通量的通量控制系数。还针对不同呼吸速率的值，针对氧化，磷酸化和质子泄漏通量计算了氧化，磷酸化和质子泄漏子系统的通量控制系数。将这些理论数据与在代谢控制分析和“自上而下”的分析方法中获得的实验结果进行比较。获得了良好的协议。在状态4中向线粒体添加少量ADP后，呼吸速率，质子动力（Delta p）和其他参数的模拟时程至少半定量地模拟了这些参数的实验测量时程。因此，可以得出结论，在当前阶段，该模型能够很好地反映各种条件下氧化磷酸化系统的不同特性，可以更深入地了解控制和调节该过程的机理，并且可用于模拟新实验，从而激发理论预测的实验验证。 [参考：46]

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