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首页> 外文期刊>Journal of neuroendocrinology >Leptin Responsive and GABAergic Projections to the Rostral Preoptic Area in Mice
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Leptin Responsive and GABAergic Projections to the Rostral Preoptic Area in Mice

机译：瘦素响应和GABA能投影到小鼠的鼻前视区。

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The adipocyte-derived hormone leptin plays a critical role in the control of reproduction via signalling in hypothalamic neurones. The drivers of the hypothalamic-pituitary-gonadal axis, the gonadotrophin-releasing hormone (GnRH) neurones, do not have the receptors for leptin. Therefore, intermediate leptin responsive neurones must provide leptin-to-GnRH signalling. We investigated the populations of leptin responsive neurones that provide input to the rostral preoptic area (rPOA) where GnRH cell bodies reside. Fluorescent retrograde tracer beads (RetroBeads; Lumafluor Inc., Naples, FL, USA) were injected into the rPOA of transgenic leptin receptor enhanced green fluorescent protein (Lepr-eGFP) reporter mice. Uptake of the RetroBeads by Lepr-eGFP neurones was assessed throughout the hypothalamus. RetroBead uptake was most evident in the medial arcuate nucleus (ARC), the dorsomedial nucleus (DMN) and the ventral premammillary nucleus (PMV) of the hypothalamus. The uptake of RetroBeads specifically by Lepr-eGFP neurones was highest in the medial ARC (18% of tracer-labelled neurones Lepr-eGFP-positive). Because neurones that are both leptin responsive and GABAergic play a critical role in the regulation of fertility by leptin, we next focussed on the location of these populations. To address whether GABAergic neurones in leptin-responsive hypothalamic regions project to the rPOA, the experiment was repeated in GABA neurone reporter mice (Vgat-tdTomato). Between 10% and 45% of RetroBead-labelled neurones in the ARC were GABAergic, whereas uptake of tracer by GABAergic neurones in the DMN and PMV was very low (<5%). These results show that both leptin responsive and GABAergic neurones from the ARC project to the region of the GnRH cell bodies. Our findings suggest that LEPR-expressing GABA neurones from the ARC may be mediators of leptin-to-GnRH signalling.
机译：脂肪细胞源性激素瘦素在下丘脑神经元中通过信号传导控制繁殖中起关键作用。下丘脑-垂体-性腺轴的驱动因子，促性腺激素释放激素（GnRH）神经元，没有瘦素受体。因此，中间的瘦素反应神经元必须提供瘦素对GnRH的信号传导。我们调查了瘦素响应神经元的人口，这些神经元向GnRH细胞体所在的延髓前视区（rPOA）提供输入。将荧光逆行示踪珠（RetroBeads; Lumafluor Inc.，那不勒斯，佛罗里达州，美国）注入转基因瘦素受体增强的绿色荧光蛋白（Lepr-eGFP）报告基因小鼠的rPOA中。在整个下丘脑中评估了Lepr-eGFP神经元对RetroBeads的摄取。 RetroBead的摄取在下丘脑的内侧弓形核（ARC），背侧核（DMN）和腹侧前乳突核（PMV）中最为明显。 Lepr-eGFP神经元对RetroBeads的摄取在内侧ARC中最高（示踪剂标记的神经元Lepr-eGFP阳性为18％）。由于瘦素反应性神经元和GABA能神经元在瘦素对生育力的调节中都起着至关重要的作用，因此我们接下来将重点放在这些人群的位置上。为了解决瘦素反应性下丘脑区域中的GABA能神经元是否投射到rPOA，在GABA神经元报告基因小鼠（Vgat-tdTomato）中重复了该实验。 ARC中RetroBead标记的神经元中有10％至45％是GABA能的，而DMN和PMV中GABA能神经元对示踪剂的吸收却非常低（<5％）。这些结果表明，来自ARC的瘦素响应神经元和GABA能神经元均投射到GnRH细胞体区域。我们的发现表明，ARC中表达LEPR的GABA神经元可能是瘦素转GnRH信号的介体。

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