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首页> 外文期刊>Biophysical Chemistry: An International Journal Devoted to the Physical Chemistry of Biological Phenomena >SMALL ANGLE NEUTRON SCATTERING FROM LYSOZYME SOLUTIONS IN UNSATURATED AND SUPERSATURATED STATES (SANS FROM LYSOZYME SOLUTIONS)
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SMALL ANGLE NEUTRON SCATTERING FROM LYSOZYME SOLUTIONS IN UNSATURATED AND SUPERSATURATED STATES (SANS FROM LYSOZYME SOLUTIONS)

机译：非饱和和超饱和状态下溶菌酶溶液的小角度中子散射（溶菌酶溶液中的Sans）

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Small angle neutron scattering (SANS) method was used to study lysozyme solutions, with particular interest in an understanding of the crystallization process at the initial stage. It is found that (1) in the unsaturated solution, the protein molecules aggregate with a continuous increase in size when NaCl concentration is increased, and (2) in the supersaturated solution, an irreversible change, superimposed on the former process, occurs when the supersaturation is realized. These facts indicate the usefulness of SANS in detecting changes of protein molecules in solution on the nanometer scale. The reliability of the SANS results are indicated by (1) comparing them with those of small angle X-ray scattering (SAXS), and (2) comparing the effect of D2O and H2O as solvent. Since the interparticle interaction is essential in the crystallization process and a simple Guinier plot analysis is not allowed, a more rigorous framework of analyzing data with interference function is developed, through which both average interparticle distance and particle size are estimated. [References: 30]
机译：小角度中子散射（SANS）方法用于研究溶菌酶溶液，尤其对了解初始阶段的结晶过程特别感兴趣。发现（1）在不饱和溶液中，当NaCl浓度增加时，蛋白质分子的尺寸会不断增大，并且（2）在过饱和溶液中，当前者的过程中会发生不可逆的变化。实现了过饱和。这些事实表明，SANS在检测纳米级溶液中蛋白质分子的变化方面很有用。通过（1）将它们与小角度X射线散射（SAXS）的结果进行比较，以及（2）比较D2O和H2O作为溶剂的效果，可以表明SANS结果的可靠性。由于粒子间的相互作用是结晶过程中必不可少的，并且不允许进行简单的吉尼尔图分析，因此建立了一个更严格的具有干扰函数的数据分析框架，据此可以估算平均粒子间距离和粒径。 [参考：30]

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