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首页> 外文期刊>Journal of Neuroimmunology: Official Bulletin of the Research Committee on Neuroimmunology of the World Federation of Neurology >Chemokine receptor expression in cultured glia and rat experimental allergic encephalomyelitis.
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Chemokine receptor expression in cultured glia and rat experimental allergic encephalomyelitis.

机译：在培养的神经胶质和大鼠实验性过敏性脑脊髓炎中趋化因子受体的表达。

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摘要

Chemokines are a group of pro-inflammatory peptides that mediate leukocyte migration and activation. Several members of the chemokine family have been shown to be synthesized by cells of the central nervous system (CNS). To begin to address the role of chemokine receptors in CNS physiology, we identified, by molecular cloning techniques, the rat orthologs of the chemokine receptors, CCR2, CCR3, CCR5, and CXCR4. CCR2 and CCR5 expression was detected in rat spleen, lung, kidney, thymus and macrophages; CCR5 mRNA was also detected in rat brain. Primary cultures of rat microglia expressed CCR5 mRNA that was regulated by IFN-gamma, while both cultured astrocytes and microglia were found to contain mRNA for CXCR4 and CX3CR1. Induction of experimental allergic encephalomyelitis (EAE) in the rat was accompanied by increased levels of CCR2, CCR5, CXCR4, and CX3CR1 mRNAs in the lumbar spinal cords of animals displaying clinical signs of the disease. These data identify the rat orthologs of chemokine receptors and demonstrate that brain, spinal cord, and cultured glial cells express chemokine receptors that can be regulated both in vitro and in vivo.
机译：趋化因子是一组介导白细胞迁移和激活的促炎肽。已显示趋化因子家族的几个成员是由中枢神经系统（CNS）的细胞合成的。为了开始探讨趋化因子受体在中枢神经系统生理中的作用，我们通过分子克隆技术鉴定了趋化因子受体，CCR2，CCR3，CCR5和CXCR4的大鼠直系同源基因。在大鼠脾，肺，肾，胸腺和巨噬细胞中检测到CCR2和CCR5的表达;在大鼠脑中也检测到CCR5 mRNA。大鼠小胶质细胞的原代培养物中表达的CCR5 mRNA受IFN-γ调节，而培养的星形胶质细胞和小胶质细胞均含有CXCR4和CX3CR1的mRNA。在大鼠中诱发实验性变应性脑脊髓炎（EAE）的同时，动物腰椎中CCR2，CCR5，CXCR4和CX3CR1 mRNA的水平升高，显示出该病的临床症状。这些数据鉴定了趋化因子受体的大鼠直系同源物，并证明脑，脊髓和培养的神经胶质细胞表达了可以在体外和体内调节的趋化因子受体。

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