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首页> 外文期刊>Journal of Neuroimmunology: Official Bulletin of the Research Committee on Neuroimmunology of the World Federation of Neurology >In vitro-staining specificity of the antibody 5-D-4 for microglia but not for monocytes and macrophages indicates that microglia are a unique subgroup of the myelomonocytic lineage.
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In vitro-staining specificity of the antibody 5-D-4 for microglia but not for monocytes and macrophages indicates that microglia are a unique subgroup of the myelomonocytic lineage.

机译：抗体5-D-4对小胶质细胞但对单核细胞和巨噬细胞没有体外染色特异性，这表明小胶质细胞是骨髓单核细胞谱系的独特亚组。

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摘要

We have previously shown that (i) the ramified phenotype and (ii) the microglia-specific pattern of membrane currents are induced not only in microglia, but also in monocytes and macrophages if they are cultured in the presence of astrocytes. These findings indicated that microglia are not a separate type of cell of the myelomonocytic lineage, but are induced to take on their unique characteristics by astrocytes. Recently, it was discovered that the antibody 5-D-4 selectively stains ramified microglia in situ. We therefore studied the influence of astrocytes and other epithelial cells on the expression of the keratan sulfate epitope recognized by 5-D-4 in microglia and other myelomonocytic cells. Our findings show that this antigen is exclusively expressed in microglia only if they are induced to ramify by coculture with either astrocytes or epithelial cells. By contrast monocytes and macrophages, even if induced to take on the ramified phenotype do not stain positive with 5-D-4. These findings indicate (i) that 5-D-4 is a specific marker for ramified microglia in vitro, and (ii) that microglia are a separate class of myelomonocytic cells, distinct from monocytes and macrophages.
机译：先前我们已经表明，（i）不仅在小胶质细胞中，而且在单核细胞和巨噬细胞中培养星形胶质细胞时，也诱导了膜电流的分枝表型和（ii）小胶质细胞特异性模式。这些发现表明，小胶质细胞不是骨髓单核细胞系的单独细胞类型，而是被星形胶质细胞诱导成具有其独特特征。最近，发现抗体5-D-4选择性地原位染色分支的小胶质细胞。因此，我们研究了星形胶质细胞和其他上皮细胞对小胶质细胞和其他骨髓单核细胞中5-D-4识别的硫酸角质素表位表达的影响。我们的发现表明，只有通过与星形胶质细胞或上皮细胞共培养诱导它们分枝，该抗原才在小胶质细胞中表达。相比之下，单核细胞和巨噬细胞即使被诱导表现出分枝表型，也不会被5-D-4染色为阳性。这些发现表明（i）5-D-4是体外分枝的小胶质细胞的特异性标记，和（ii）小胶质细胞是与单核细胞和巨噬细胞不同的另一类骨髓单核细胞。

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