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首页> 外文期刊>Journal of Neuroimmunology: Official Bulletin of the Research Committee on Neuroimmunology of the World Federation of Neurology >Anti-GM(2) IgM antibody-induced complement-mediated cytotoxicity in patients with dysimmune neuropathies.
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Anti-GM(2) IgM antibody-induced complement-mediated cytotoxicity in patients with dysimmune neuropathies.

机译：抗GM（2）IgM抗体诱导的免疫缺陷性神经病患者的补体介导的细胞毒性。

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摘要

Anti-GM2 IgM antibodies have been reported in some patients with dysimmune neuropathy or lower motor neuron syndrome. To determine whether these antibodies can induce complement-dependent cytolysis we performed a cytotoxicity assay on neuroblastoma cells with sera from seven patients with demyelinating dysimmune neuropathies and high titers of anti-GM2 IgM. As controls we used sera from seven patients with other anti-neural reactivities, six with the same neuropathies but no anti-GM2 or other anti-neural reactivity and from eight normal subjects. Of the seven positive sera tested, six induced complement-mediated cytotoxicity, while none of the controls had any relevant effect on neuroblastoma cells. Preincubation of positive sera with purified GM2 removed cytotoxic activity. Affinity purified anti-GM2 IgM had the same cytotoxic anti-GM2 effect of whole serum while serum or complement alone did not have any effect. In four anti-GM2-positive patients the percentage of cell lysis correlated with anti-GM2 titers and with IgM staining of neuroblastoma cells while in two the cytotoxic effect was higher than expected from antibody titers. Complement-mediated cell lysis induced by anti-GM2 IgM antibodies may be a possible mechanism of neural damage in patients with dysimmune neuropathy and high titers of anti-GM2 IgM antibodies.
机译：抗GM2 IgM抗体已在一些免疫功能低下的神经病或下运动神经元综合征的患者中报道。为了确定这些抗体是否可以诱导补体依赖性细胞溶解，我们对来自7名脱髓鞘性免疫不良神经病和高滴度抗GM2 IgM患者的血清神经母细胞瘤细胞进行了细胞毒性测定。作为对照，我们使用了七名具有其他抗神经反应性的患者，六名具有相同神经病但无抗GM2或其他抗神经反应性的患者以及八名正常受试者的血清。在测试的七个阳性血清中，六个诱导了补体介导的细胞毒性，而对照组均没有对神经母细胞瘤细胞产生任何相关影响。阳性血清与纯化的GM2的预温育可去除细胞毒活性。亲和纯化的抗GM2 IgM具有与整个血清相同的细胞毒性抗GM2效应，而单独的血清或补体则没有任何作用。在四名抗GM2阳性的患者中，细胞裂解的百分比与抗GM2滴度和成神经细胞瘤细胞的IgM染色相关，而在两个中，细胞毒作用高于抗体滴度的预期。由抗GM2 IgM抗体诱导的补体介导的细胞裂解可能是免疫功能异常神经病和高滴度抗GM2 IgM抗体患者神经损伤的可能机制。

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