首页> 外文期刊>Journal of Neuroimmunology: Official Bulletin of the Research Committee on Neuroimmunology of the World Federation of Neurology >Regulation of prolactin expression in leukemic cell lines and peripheral blood mononuclear cells.
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Regulation of prolactin expression in leukemic cell lines and peripheral blood mononuclear cells.

机译:泌乳素在白血病细胞系和外周血单核细胞中表达的调节。

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摘要

To address the role of different intracellular signals in prolactin (PRL) expression in leukocytes, we have investigated the effects of chlorophenylthio-cAMP (cptcAMP), phorbol myristate acetate (PMA) and ionomycin on the activation of the upstream PRL promoter in several leukemic cell lines. All three stimulators, alone or in synergism with each other, were able to modulate promoter activity, but their actions were cell-type dependent. In freshly isolated peripheral blood mononuclear cells (PBMC), PRL expression could only be stimulated by cptcAMP. The physiological importance of cAMP in the regulation of PRL expression in leukocytes is suggested by the finding that in PBMC, PRL expression is enhanced by prostaglandin-E(2) and the beta(2)-adrenergic agonist terbutaline, which both signal through cAMP.
机译:为解决不同细胞内信号在白细胞催乳素(PRL)表达中的作用,我们研究了氯苯硫基-cAMP(cptcAMP),佛波肉豆蔻酸乙酸酯(PMA)和离子霉素对几种白血病细胞中上游PRL启动子活化的影响线。三种单独的刺激物或彼此协同作用都能够调节启动子活性,但它们的作用取决于细胞类型。在新鲜分离的外周血单核细胞(PBMC)中,PRL表达只能被cptcAMP刺激。发现在PBMC中,前列腺素-E(2)和β(2)-肾上腺素能激动剂特布他林会增强cAMP在白细胞中PRL表达的调节中的生理重要性,这两者均通过cAMP发出信号。

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