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首页> 外文期刊>Journal of Neuroimmunology: Official Bulletin of the Research Committee on Neuroimmunology of the World Federation of Neurology >Human antibody-dependent cellular cytotoxicity mediated by interferon gamma-activated neutrophils is impaired by vasoactive intestinal peptide.
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Human antibody-dependent cellular cytotoxicity mediated by interferon gamma-activated neutrophils is impaired by vasoactive intestinal peptide.

机译：干扰素γ激活的中性粒细胞介导的人抗体依赖性细胞毒性被血管活性肠肽削弱。

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摘要

The aim of the present study was to evaluate the effect of vasoactive intestinal peptide (VIP) on the expression and activity of receptors for the Fc portion of IgG (Fc gamma R) in human neutrophils. Cells were assayed under basal conditions and following in vitro stimulation with interferon gamma (IFN gamma). Antibody dependent-cellular cytotoxicity (ADCC) was chosen as a means of evaluating Fc gamma R activity. The results indicated that incubation with VIP (10(-6) M) during 18 h slightly diminished cytotoxicity of non stimulated neutrophils. In contrast, VIP exerted a marked inhibitory effect on neutrophils activated with IFN gamma. Similar results were obtained with forskolin, another agent that increases intracellular cAMP. Finally, using monoclonal antibodies and flow cytometry analysis, we found decreased membrane expression of Fc gamma R after VIP incubation. Taken together, these results show that VIP is able to act on human neutrophils, partially blocking IFN gamma-activation of Fc gamma R mediated functions. Modulation of neutrophil cytotoxic response by VIP may have an important role in limiting tissue injury during inflammation.
机译：本研究的目的是评估血管活性肠肽（VIP）对人嗜中性白细胞中IgG Fc部分（Fc gamma R）受体表达和活性的影响。在基础条件下并在用干扰素γ（IFNγ）体外刺激后测定细胞。选择抗体依赖性细胞毒性（ADCC）作为评估FcγR活性的手段。结果表明，在18小时内与VIP（10（-6）M）孵育会略微降低非刺激性中性粒细胞的细胞毒性。相反，VIP对被IFNγ激活的嗜中性白细胞具有明显的抑制作用。用福司可林（一种增加细胞内cAMP的药物）获得了相似的结果。最后，使用单克隆抗体和流式细胞仪分析，我们发现VIP孵育后FcγR的膜表达降低。综上所述，这些结果表明VIP能够作用于人类嗜中性粒细胞，部分阻断FcγR介导的功能的IFNγ活化。 VIP调节中性粒细胞的细胞毒性反应可能在限制炎症过程中的组织损伤中起重要作用。

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