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首页> 外文期刊>Journal of Neuroimmunology: Official Bulletin of the Research Committee on Neuroimmunology of the World Federation of Neurology >Increased delayed type hypersensitivity in rats subjected to unilateral mononeuropathy is mediated by neurokinin-1 receptors.
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Increased delayed type hypersensitivity in rats subjected to unilateral mononeuropathy is mediated by neurokinin-1 receptors.

机译：单侧单神经病大鼠的迟发型超敏反应增加是由神经激肽1受体介导的。

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摘要

An animal model or peripheral mononeuropathy was utilized in the present study to investigate the potential role of substance P (SP) in modifying immune responses associated with chronic pain conditions. Animals subjected to unilateral sciatic ligation and sham-operated animals were sensitized with keyhole limpet hemocyanin (KLH) and subsequently challenged in the ipsilateral or contralateral hind paw to produce a delayed-type hypersensitivity (DTH) response. Subcutaneous microdialysis and radioimmunoassay were used to measure interstitial fluid SP levels in the challenged tissue prior to and following immune challenge in control and neuropathic animals. Following immune challenge, there was a significant increase in the concentration of SP in tissue dialysate samples from the challenged paw of both sham-operated and neuropathic animals. However, tissue SP levels in neuropathic animals were more than two-fold higher than those obtained from sham-operated controls following challenge. SP concentration remained elevated for 2.5 h following immune challenge in neuropathic animals compared to 90 min in sham-operated animals. Compared with controls, neuropathic animals also exhibited an increased DTH response that was reversed, in a dose-related fashion, by the non-peptide NK-1 receptor blocker L-703,606. The same antagonist had no effect in sham-operated animals. These data suggest that the increased DTH response in animals subjected to unilateral mononeuropathy involves SP and NK-1 receptors present in the challenged tissue.
机译：在本研究中，使用动物模型或周围性单神经病来研究P物质（SP）在改变与慢性疼痛状况相关的免疫反应中的潜在作用。将接受单侧坐骨结扎的动物和假手术的动物用匙孔血蓝蛋白（KLH）致敏，随后在同侧或对侧后爪中激发以产生迟发型超敏反应（DTH）。在对照和神经病动物进行免疫攻击之前和之后，皮下微透析和放射免疫分析法被用来测量被攻击组织中的间质液SP水平。免疫攻击后，假手术和神经性动物的攻击爪的组织透析液样品中SP的浓度显着增加。但是，神经病动物的组织SP水平比攻击后从假手术对照中获得的组织SP水平高两倍以上。在神经性动物免疫攻击后，SP浓度在2.5小时内保持升高，而假手术动物则为90分钟。与对照组相比，神经病动物还表现出DTH反应增加，这种反应以剂量相关的方式被非肽NK-1受体阻滞剂L-703,606逆转。相同的拮抗剂在假手术动物中没有作用。这些数据表明，在遭受单侧单神经病的动物中，DTH反应增加涉及受攻击组织中存在的SP和NK-1受体。

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