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首页> 外文期刊>Journal of Neuroimmunology: Official Bulletin of the Research Committee on Neuroimmunology of the World Federation of Neurology >IL-6 and TNFalpha expression in brains of twitcher, quaking and normal mice.
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IL-6 and TNFalpha expression in brains of twitcher, quaking and normal mice.

机译：抽搐，地震和正常小鼠的大脑中IL-6和TNFalpha的表达。

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摘要

Cytokines have been postulated to play a pathogenic role in twitcher mice, which are an animal model of globoid cell leukodystrophy. In particular, TNFalpha promotes oligodendrocyte and myelin pathology, and IL-6 expression is induced in astrocyte and microglial cultures that have been incubated with TNFalpha or myelin debris, respectively. It is unknown whether these cytokines are expressed in twitcher mice. The objectives of the present study were to develop an immunohistochemical method to detect TNFalpha and IL-6 in the mouse CNS, and then utilize this method to identify the cell types expressing these cytokines, and their spatial distribution, in the brains of normal, twitcher and quaking mice. In normal mice, IL-6 was found in ependymal cells, Bergmann glia, in processes that were adjacent or attached to the ventricles or pial surface, and in lightly stained processes in white matter. These processes were identified to belong to astrocytes and microglia. IL-6 staining was dramatically increased in twitcher mice. Astrocytes, with reactive features, and microglia were labeled in the cerebral cortex, basal ganglia, subcortical white matter, pons, medulla and cerebellar white matter. IL-6-positive reactive astrocytes were less abundant in quaking mice than twitcher mice. Cells expressing TNFalpha were rare or absent in normal and quaking mice. In twitcher mice, TNFalpha-positive macrophages were present at a lower concentration in cerebral white matter than in the pons and medulla, which have more advanced demyelination. These data demonstrate that pathological events induce the expressions of TNFalpha and IL-6 in the CNS of twitcher mice.
机译：已经假定细胞因子在抽搐小鼠中发挥致病作用，抽搐小鼠是球形细胞白细胞营养不良的动物模型。特别地，TNFα促进少突胶质细胞和髓鞘病理，并且IL-6表达在分别与TNFα或髓鞘碎片孵育的星形胶质细胞和小胶质细胞培养物中被诱导。这些细胞因子是否在抽搐小鼠中表达尚不清楚。本研究的目的是开发一种免疫组织化学方法来检测小鼠CNS中的TNFalpha和IL-6，然后利用该方法鉴定正常，细颈动物脑中表达这些细胞因子的细胞类型及其空间分布。和震颤的老鼠。在正常小鼠中，在室管膜细胞，Bergmann胶质细胞，邻近或附着于心室或脑膜表面的过程以及白质中的浅色染色过程中发现了IL-6。这些过程被确定为属于星形胶质细胞和小胶质细胞。在抽搐小鼠中，IL-6染色显着增加。在大脑皮层，基底神经节，皮质下白质，脑桥，延髓和小脑白质中标记具有反应性特征的星形胶质细胞和小胶质细胞。 IL-6阳性反应性星形胶质细胞在地震小鼠中不如抽搐小鼠丰富。在正常和震颤小鼠中，表达TNFα的细胞很少或不存在。在抽搐小鼠中，TNFα阳性巨噬细胞在脑白质中的浓度低于在脑桥白质中具有更高脱髓鞘作用的脑桥和延髓中的浓度。这些数据证明病理事件诱导了抽搐小鼠中枢神经系统中TNFα和IL-6的表达。

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