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首页> 外文期刊>Journal of Neuroimmunology: Official Bulletin of the Research Committee on Neuroimmunology of the World Federation of Neurology >CD59 homologue regulates complement-dependent cytolysis of rat Schwann cells.
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CD59 homologue regulates complement-dependent cytolysis of rat Schwann cells.

机译：CD59同源物调节大鼠雪旺细胞的补体依赖性细胞溶解。

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摘要

Antibody (Ab) sensitized sciatic nerve Schwann cells (SchC) of 2-day-old rats (SchC/2d) were significantly more susceptible to cytolysis by both heterologous, guinea pig (GP), and homologous rat serum complement (40 +/- 3.8% and 21.2 +/- 3.1%, respectively) than SchC of 6-day-old rats (SchC/6d) (7.9 +/- 5.9% and 2.6 +/- 3.1%, respectively). To determine if resistance to complement (C)-mediated cytolysis correlated with expression of membrane proteins which regulate C activation, we used Western blot and FACS analysis. Binding of specific polyclonal Ab demonstrated similar concentrations of Crry, a regulator of C3 convertase formation, on plasma membranes of SchC 2d and 6d. During C activation, both C3b deposition and iC3b formation were greater on SchC/6d than on SchC/2d and the C3b deposition did not correlate with enhanced cytolysis. In contrast, 2.1-fold more rat CD59, a regulator of C8 and C9 incorporation into C5b-9, detected with Western blot on SchC/6d compared with SchC/2d was confirmed by FACS. Further, both rat and GP C8/C9 lysed SchC/2d expressing human C5b-7 (20.1 +/- 3.7 and 21.6 +/- 4.7%, respectively), while only GP C8/C9 caused cytolysis of 10.7 +/- 4.3% SchC/6d expressing hu C5b-7 and rat C8/C9 did not (0.5 +/- 0.5%). Preincubation of SchC/6d with an F(ab)2 fragment of an mAb to rCD59 with blocking capacity, increased cytolysis mediated by rat serum C more than 6-fold to 16.7 +/- 3.0% but only 1.7-fold (maximum cytolysis 37.4 +/- 11.2%) in SchC/2d. Our data suggest that expression of rat CD59 on SchC increased almost two-fold between postnatal days 2 and 6, and this increased expression on more terminally differentiated SchC is a significant factor in regulating terminal complement complex formation and limiting cytolysis of rat SchC by homologous serum complement.
机译：2天大的大鼠（SchC / 2d）的抗体（Ab）致敏的坐骨神经雪旺细胞（SchC）对异源豚鼠（GP）和同源大鼠血清补体的溶胞作用明显更高（40 +/-比6日龄大鼠的SchC（SchC / 6d）分别高3.8％和21.2 +/- 3.1％（分别为7.9 +/- 5.9％和2.6 +/- 3.1％）。为了确定对补体（C）介导的细胞溶解的抗性是否与调节C活化的膜蛋白表达相关，我们使用了蛋白质印迹和FACS分析。特异性多克隆抗体的结合在SchC 2d和6d的质膜上显示出类似浓度的Crry，C3转化酶形成的调节剂。在C激活过程中，SchC / 6d上的C3b沉积和iC3b的形成均大于SchC / 2d上的C3b沉积，并且C3b的沉积与细胞溶解增强无关。相反，通过FACS证实，在SchC / 6d上用Western blot检测到的大鼠CD59（C8和C9掺入C5b-9的调节剂）比SchC / 2d多了2.1倍。此外，大鼠和GP C​​8 / C9都裂解了表达人C5b-7的SchC / 2d（分别为20.1 +/- 3.7和21.6 +/- 4.7％），而只有GP C8 / C9引起了10.7 +/- 4.3％的细胞溶解表达hu C5b-7和大鼠C8 / C9的SchC / 6d不表达（0.5 +/- 0.5％）。将SchC / 6d与mAb的F（ab）2片段预孵育至具有阻断能力的rCD59，大鼠血清C介导的细胞溶解增加6倍以上至16.7 +/- 3.0％，但仅为1.7倍（最大细胞溶解37.4） +/- 11.2％）在SchC / 2d中。我们的数据表明，在出生后第2天和第6天之间，SchC上大鼠CD59的表达几乎增加了两倍，而在终末分化的SchC上这种表达的增加是调节终末补体复合物形成和限制同源血清限制大鼠SchC细胞溶解的重要因素补充。

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