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首页> 外文期刊>Journal of Neuroimmunology: Official Bulletin of the Research Committee on Neuroimmunology of the World Federation of Neurology >SV40 large T immortalised cell lines of the rat blood-brain and blood-retinal barriers retain their phenotypic and immunological characteristics.
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SV40 large T immortalised cell lines of the rat blood-brain and blood-retinal barriers retain their phenotypic and immunological characteristics.

机译：SV40大T永生的大鼠血脑细胞和血视网膜屏障细胞系保留其表型和免疫学特征。

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摘要

In the central nervous system the blood-brain and blood-retinal barriers (BBB and BRB respectively) are instrumental in maintaining homeostasis of the neural parenchyma and controlling leucocyte traffic. These cellular barriers are formed primarily by the vascular endothelium of the brain and retina although in the latter the pigmented epithelial cells also form part of the barrier. From primary cultures of rat brain endothelium, retinal endothelium and retinal pigment epithelium (RPE) we have generated temperature sensitive SV40 large T immortalised cell lines. Clones of brain (GP8.3) and retinal (JG2.1) endothelia and RPE (LD7.4) have been derived from parent lines that express the large T antigen at the permissive temperature. The endothelial cell (EC) lines expressed P-glycoprotein, GLUT-1, the transferrin receptor, von Willebrand factor and the RECA-1 antigen and exhibited high affinity uptake of acetylated LDL and stained positive with the lectin Griffonia simplicifolia. The RPE cell line was positive for cytokeratins and for the rat RPE antigen RET-PE2. All the cell lines expressed major histocompatibility complex (MHC) class 1 and intercellular adhesion molecule (ICAM)-1 constitutively and could be induced to express MHC class II and vascular cell adhesion molecule (VCAM)-1 following cytokine activation. The EC also expressed platelet endothelial cell adhesion molecule (PECAM)-1. Monolayers of these cells could support the migration of antigen-specific T cell lines. The generation of immortalised cell lines derived from the rat BBB and BRB should prove to be useful tools for the study of these specialised cellular barriers.
机译：在中枢神经系统中，血脑屏障和血视网膜屏障（分别为BBB和BRB）在维持神经实质的稳态和控制白细胞流量方面发挥了作用。这些细胞屏障主要由大脑和视网膜的血管内皮形成，尽管在后者中，色素上皮细胞也形成了屏障的一部分。从大鼠脑内皮，视网膜内皮和视网膜色素上皮（RPE）的原代培养物中，我们产生了对温度敏感的SV40大T永生化细胞系。脑（GP8.3）和视网膜（JG2.1）内皮和RPE（LD7.4）的克隆已衍生自在允许温度下表达大T抗原的亲本系。内皮细胞（EC）系表达P-糖蛋白，GLUT-1，转铁蛋白受体，von Willebrand因子和RECA-1抗原，并表现出对乙酰化LDL的高亲和力吸收，并被凝集素Griffonia simplicifolia染色呈阳性。 RPE细胞系对细胞角蛋白和大鼠RPE抗原RET-PE2呈阳性。所有细胞系均组成性表达主要组织相容性复合物（MHC）1类和细胞间粘附分子（ICAM）-1，并在细胞因子激活后可诱导表达MHC II类和血管细胞粘附分子（VCAM）-1。 EC还表达了血小板内皮细胞粘附分子（PECAM）-1。这些细胞的单层可以支持抗原特异性T细胞系的迁移。源自大鼠BBB和BRB的永生细胞系的生成应被证明是研究这些专门细胞屏障的有用工具。

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