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首页> 外文期刊>Journal of Neuroimmunology: Official Bulletin of the Research Committee on Neuroimmunology of the World Federation of Neurology >Identification of cell types producing RANTES, MIP-1 alpha and MIP-1 beta in rat experimental autoimmune encephalomyelitis by in situ hybridization.
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Identification of cell types producing RANTES, MIP-1 alpha and MIP-1 beta in rat experimental autoimmune encephalomyelitis by in situ hybridization.

机译：通过原位杂交鉴定大鼠实验性自身免疫性脑脊髓炎中产生RANTES，MIP-1 alpha和MIP-1 beta的细胞类型。

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摘要

The chemokines RANTES, macrophage inflammatory protein (MIP)-1 alpha and MIP-1 beta are members of the beta-family of chemokines and potent chemoattractants for lymphocytes and monocytes. To investigate the factors which regulate lymphocyte traffic in experimental autoimmune encephalomyelitis (EAE), we studied, by in situ hybridization analysis, the kinetics of mRNA expression and the potent cellular sources of RANTES, MIP-1 alpha and MIP-1 beta in the central nervous system (CNS) during the course of EAE. RANTES-positive cells appeared in the subarachnoid space and infiltrated the subpial region at around day 10, increased to a peak at days 12-13 and then decreased following the resolution of the acute phase of EAE, though elevated RANTES message expressions still remained on chronic subclinical stage. Most of RANTES positive cells were identified as T-lymphocytes located mainly around blood vessels, by combined studies of in situ hybridization and immunohistochemistry. The remainder of the RANTES-positive cells were astrocytes and macrophages/microglia. MIP-1 alpha and MIP-1 beta mRNA-positive cells appeared around day 10, increased further on days 12-13 and then gradually decreased. Most of the MIP-1 alpha- and MIP-1 beta-positive mononuclear cells were located around blood vessels. The kinetics of RANTES, MIP-1 alpha and MIP-1 beta expression paralleled those of the recruitment of infiltrating inflammatory cells and disease severity. Our observations support the possibility that chemokine production by T-cells, macrophages and astrocytes lead to the infiltration of inflammatory cells into the CNS parenchyma during the acute phase of EAE.
机译：趋化因子RANTES，巨噬细胞炎性蛋白（MIP）-1 alpha和MIP-1 beta是趋化因子β家族的成员，并且是淋巴细胞和单核细胞的有效趋化因子。为了研究在实验性自身免疫性脑脊髓炎（EAE）中调节淋巴细胞流量的因素，我们通过原位杂交分析研究了中枢区域RANTES，MIP-1 alpha和MIP-1 beta的mRNA表达动力学和强大的细胞来源EAE过程中的神经系统（CNS）。 RANTES阳性细胞出现在蛛网膜下腔中，并在第10天左右浸润到蛛网膜下区域，在第12-13天增加到峰值，然后在EAE急性期消退后减少，尽管在慢性患者中仍会升高RANTES信息表达亚临床阶段。通过原位杂交和免疫组织化学的联合研究，大多数RANTES阳性细胞被鉴定为主要位于血管周围的T淋巴细胞。其余的RANTES阳性细胞是星形胶质细胞和巨噬细胞/小胶质细胞。 MIP-1α和MIP-1βmRNA阳性细胞在第10天左右出现，在第12-13天进一步增加，然后逐渐减少。大多数MIP-1α和MIP-1β阳性单核细胞位于血管周围。 RANTES，MIP-1α和MIP-1β表达的动力学与浸润性炎症细胞募集和疾病严重程度的动力学相似。我们的观察结果支持了在EAE急性期T细胞，巨噬细胞和星形胶质细胞产生趋化因子导致炎症细胞浸入CNS实质的可能性。

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