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首页> 外文期刊>Journal of Neuroimmunology: Official Bulletin of the Research Committee on Neuroimmunology of the World Federation of Neurology >Expression of an alpha7 duplicate nicotinic acetylcholine receptor-related protein in human leukocytes.
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Expression of an alpha7 duplicate nicotinic acetylcholine receptor-related protein in human leukocytes.

机译：alpha7复制烟碱型乙酰胆碱受体相关蛋白在人白细胞中的表达。

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摘要

We examined the potential expression and function of alpha7 nicotinic acetylcholine receptors (nAChRs) in leukocytes. RT-PCR with alpha7 specific primers revealed the presence of the receptor mRNA in leukocytes. Immunoblotting and immunofluorescence experiments demonstrated the expression of a protein that is recognized by alpha7 specific antibodies. However, nicotine and acetylcholine (ACh) failed to elicit current in leukocytes. Binding experiments with alpha-bungarotoxin rhodamine conjugated were negative, illustrating the absence of a high-affinity binding site. RT-PCR analysis revealed the selective expression of the dupalpha7 mRNA. These data indicate that leukocytes express in their membrane the dupalpha7 protein but its physiological role remains to be identified.
机译：我们检查了白细胞中α7烟碱乙酰胆碱受体（nAChRs）的潜在表达和功能。使用alpha7特异性引物的RT-PCR显示白细胞中受体mRNA的存在。免疫印迹和免疫荧光实验证明了被alpha7特异性抗体识别的蛋白质的表达。但是，尼古丁和乙酰胆碱（ACh）未能在白细胞中引发电流。结合了α-真菌毒素若丹明的结合实验是阴性的，说明没有高亲和力的结合位点。 RT-PCR分析显示了dupalpha7 mRNA的选择性表达。这些数据表明白细胞在其膜中表达dupalpha7蛋白，但其生理作用尚待确定。

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