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首页> 外文期刊>Journal of Microencapsulation: Microcapsules Liposomes Nanoparticles Microcells Microspheres >Physicochemical characterization of protamine-phosphorothioate nanoparticles
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Physicochemical characterization of protamine-phosphorothioate nanoparticles

机译：鱼精蛋白-硫代磷酸酯纳米颗粒的理化特性

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Protamine-oligonucleotide nanoparticles represent effective colloidal drug carriers for antisense phosphorothioate oligonucleotides (PTO).This study describes improvements in particle preparation and the physicochemical properties of the complexes prepared.The influence of component concentrations,length of the PTO chain and the PTO/protamine weight ratio on particle formation and size,shape and surface charge of the particles were studied in detail.Nanoparticles with diameters of 90-200 nm were obtained,using prot-amine free base (PFB) and phosphorothioate in water.The chemical composition of the nanoparticles was analysed.More than 90% of the PTO could be assembled in the particle matrix using a >1:2 ratio (w/w) of PTO and PFB.About 53-68% of the PFB was incorporated in the particle matrix.The complexes had a zetapotential of -19 up to +32mV,depending on the PTO/PFB ratio.The kinetics of the assembly of this binary system were observed by dynamic light scattering (DLS) measurements and by sedimentation velocity analysis in the analytical ultracentrifuge (AUC).In addition,scanning electron microscopy (SEM) and atomic force microscopy (AFM) were applied to verify .the results of DLS and the ultracentrifuge measurements.According to sedimentation velocity analysis,the particles were only moderately stable in water and unstable in salt solutions.However,the colloidal solution in water could be stabilized by polyethylenglycol 20000 (PEG),which also led to an increase of stability in cell medium.
机译：鱼精蛋白-寡核苷酸纳米颗粒代表了有效的胶体药物载体，可用于反义硫代磷酸酯寡核苷酸（PTO）。这项研究描述了颗粒制备和制备的复合物的理化性质方面的改进。组分浓度，PTO链长度和PTO /鱼精蛋白重量的影响详细研究了颗粒的形成比例，粒径，形状和表面电荷。利用鱼精蛋白游离碱（PFB）和硫代磷酸酯制备了直径为90-200 nm的纳米颗粒。经过分析，PTO和PFB的比率（w / w）> 1：2（w / w）可以将超过90％的PTO组装在颗粒基质中，约53-68％的PFB被掺入颗粒基质中。取决于PTO / PFB比，复合物的Zetapotential为-19，最高+ 32mV。通过动态光散射（DLS）测量来观察该二元体系的组装动力学。在分析型超速离心机（AUC）中进行沉降速度分析。此外，采用扫描电子显微镜（SEM）和原子力显微镜（AFM）验证DLS的结果和超速离心机的测量结果。粒子在水中仅能适度稳定，而在盐溶液中则不稳定。但是，聚乙二醇20000（PEG）可以使水中的胶体溶液稳定，这也增加了细胞培养基的稳定性。

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