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首页> 外文期刊>Journal of Nematology, with Annual of Applied Nematology >The role of wood-inhabiting bacteria in pine wilt disease
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The role of wood-inhabiting bacteria in pine wilt disease

机译：居住木材的细菌在松萎病中的作用

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摘要

The pathogenicity of the pine wood nematode (PWN), Bursaphelenchus xylophilus together with the bacteria isolated from black pine (Pinus thunbergii) bark inoculated to axenic black pine seedlings, significantly exceeded that of the axenic PWNs alone, demonstrating that the bacteria play an important role in pine wilt disease. Inoculation of seedlings with bacteria-free culture filtrates of the seven isolates from the dead seedlings from the above experiment showed that all isolate filtrates killed the seedlings within 8 days. Identification of the bacteria using 16S rDNA sequencing showed that the isolates belonged to strains By253Ydz-fq, S209, 210-50 and 210-50 in Bacillus and the DN1.1 strain of Stenotrophomonas mallophilia, respectively. Completing Koch's postulates using the seven bacterial isolates to inoculate pine seedlings showed that all the seedlings that received aseptic PWNs mixed with the seven bacterial isolates died within 18 days post inoculation, while those inoculated with 'wild PWNs died 16 days post inoculation. No disease symptoms developed on seedlings that received sterile water or aseptic PWNs. The horizontal transfer of the pathogenic bacteria may explain differences in bacterial species carried by PWN in different geographic areas.
机译：松木线虫（PWN），松材线虫（Bursaphelenchus xylophilus）以及从黑松（Pinus thunbergii）树皮中分离出的细菌的致病性大大超过了仅接种过木本PWNs的木本线虫，这表明细菌在其中发挥了重要作用在松萎病中。根据上述实验，从死苗的七株分离物中接种无细菌的培养液，然后接种幼苗，结果表明所有分离物的滤液在8天内杀死了幼苗。使用16S rDNA测序鉴定细菌表明，分离株分别属于芽孢杆菌中的By253Ydz-fq，S209、210-50和210-50菌株和嗜麦芽窄食单胞菌的DN1.1菌株。用7种细菌分离株接种松树苗来完成Koch的假设表明，所有接受了无菌PWN与7种细菌分离株混合的苗在接种后18天内死亡，而接种“野生PWN”的幼苗在接种后16天死亡。接受无菌水或无菌PWN的幼苗没有病害症状。病原菌的水平转移可以解释PWN在不同地理区域携带的细菌种类的差异。

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