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首页> 外文期刊>Journal of Nematology, with Annual of Applied Nematology >Scanning Electron Microscopy of Pine Seedling Wood Tissue Sections Inoculated with the Pinewood Nematode Bursaphelenchus xylophilus Previously Prepared for Light Microscopy
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Scanning Electron Microscopy of Pine Seedling Wood Tissue Sections Inoculated with the Pinewood Nematode Bursaphelenchus xylophilus Previously Prepared for Light Microscopy

机译：先前准备用于光学显微镜的松木线虫Bursaphelenchus xylophilus接种的松树幼苗木组织切片的扫描电子显微镜

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Scanning electron microscopy (SEM) was applied to paraffin-embedded wood sections to study the histopathology of pine seedlings inoculated with the pinewood nematode (PWN), Bursaphelenchus xylophilus. The sections, which had been previously prepared and observed by light microscopy (LM) on glass slides, were originally obtained from experiments in which pine seedlings had been inoculated with PWN. The cover glass was removed by soaking the glass slide in xylene for 3 to 5 days. The glass slides werecut into small pieces so that each piece contained one wood section. Each piece of the glass slide was attached with double adhesive tape to an aluminum stub. The specimens were sputter-coated with gold and examined with a scanning electron microscope (JEOL-JSM 5200). Compared to LM (as documented in previous reports) SEM provided greater depth of focus and resolution of the damaged wood tissues, nematodes and associated bacteria. SEM made it possible to observe the relationship between bacterial distribution and nematode distribution in wood tissues. SEM observations also suggested the possibility of documenting the death of ray cells and other parenchyma cells in relation to disease development. Finally, the current study of PWN in pine seedlings demonstrated that glass slides prepared for LM observations more than 25 years earlier could be successfully processed for examination by SEM.
机译：将扫描电子显微镜（SEM）应用于石蜡包埋的木材切片，以研究接种松木线虫（PWN），松材线虫的松树幼苗的组织病理学。这些切片是先前准备并通过光学显微镜（LM）在载玻片上观察到的切片，最初是从已用PWN接种松树苗的实验中获得的。通过将载玻片浸入二甲苯中3至5天来除去保护玻璃。将载玻片切成小块，以便每块包含一个木块。将玻璃载玻片的每一块均用双面胶带附接到铝制短管上。将样品溅射镀金，并用扫描电子显微镜（JEOL-JSM 5200）检查。与LM（如以前的报告中所述）相比，SEM可以提供更大的聚焦深度和对受损的木质组织，线虫和相关细菌的分辨率。 SEM使观察木质组织中细菌分布和线虫分布之间的关系成为可能。 SEM观察还表明，有可能记录与疾病发展相关的射线细胞和其他实质细胞的死亡。最后，目前对松树幼苗中PWN的研究表明，为25年前的LM观测而准备的载玻片可以成功加工以进行SEM检查。

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