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首页> 外文期刊>Journal of Microencapsulation: Microcapsules Liposomes Nanoparticles Microcells Microspheres >Collagen-coated polycaprolactone microparticles as a controlled drug delivery system
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Collagen-coated polycaprolactone microparticles as a controlled drug delivery system

机译：胶原蛋白包衣的聚己内酯微粒作为药物控制系统

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Objective: Polycaprolactone (PCL) microparticles coated with acetylated collagen have been assessed for use as a controlled drug delivery system. Method: The surface morphology, drug encapsulation and release profile of PCL microparticles and collagen-coated PCL microparticles containing doxycycline hydrochloride (DH) have been investigated in order to develop a controlled release system which would in addition act as a scaffold for cell attachment. PCL microparticles were prepared by emulsion solvent evaporation technique and loaded with DH. Since the encapsulation was found to be low, PCL microparticles were coated with acetylated collagen containing DH, to increase the drug availability. Collagen was modified by acetylation to shift its isoelectric point and to have acetylated collagen solution at pH 7.0. The microparticles were characterized using a scanning electron microscope (SEM) and the in vitro drug release profile was determined using HPLC. Results: Uniform sized (～1000nm) PCL microparticles were prepared using 4% PVA in the external water phase. Acetylated collagen at pH 7.0 was coated onto the PCL microparticles. This resulted in microparticles of uniform size at neutral pH. PCL acts as a support for collagen which acts as a scaffold for cell attachment. In vitro drug release studies show that collagen-coated PCL microparticle is a promising candidate for controlled drug delivery system having release duration of over 10 days. In vitro fibroblast culture studies reveal that collagen is a good substrate for cell attachment and would provide a stable environment for cell proliferation and regeneration. Thus, this system would be ideal for a short-term drug delivery to create an aseptic environment where cells can adhere and proliferate to regenerate the site.
机译：目的：已评估了涂有乙酰化胶原蛋白的聚己内酯（PCL）微粒可作为受控药物输送系统。方法：已研究了含有盐酸多西环素（DH）的PCL微粒和胶原蛋白包被的PCL微粒的表面形态，药物包封和释放特性，以开发控释系统，该系统还可作为细胞附着的支架。通过乳液溶剂蒸发技术制备PCL微粒并负载DH。由于发现包封率低，因此将PCL微粒涂上含DH的乙酰化胶原蛋白，以增加药物利用率。胶原蛋白通过乙酰化作用进行修饰，以改变其等电点并在pH 7.0下具有乙酰化的胶原蛋白溶液。使用扫描电子显微镜（SEM）表征微粒，并使用HPLC确定体外药物释放曲线。结果：在外部水相中使用4％PVA制备了均一尺寸（〜1000nm）的PCL微粒。将pH 7.0的乙酰化胶原涂覆在PCL微粒上。这导致在中性pH下尺寸均匀的微粒。 PCL充当胶原蛋白的支持物，而胶原蛋白充当细胞附着的支架。体外药物释放研究表明，胶原包衣的PCL微粒是释放时间超过10天的受控药物输送系统的有希望的候选者。体外成纤维细胞培养研究表明，胶原蛋白是细胞附着的良好底物，可为细胞增殖和再生提供稳定的环境。因此，该系统对于短期药物输送以创造无菌环境是理想的，在该环境中细胞可以粘附并增殖以再生该部位。

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