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首页> 外文期刊>Journal of Microencapsulation: Microcapsules Liposomes Nanoparticles Microcells Microspheres >Development of bioadhesive amino-pegylated poly(anhydride) nanoparticles designed for oral DNA delivery
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Development of bioadhesive amino-pegylated poly(anhydride) nanoparticles designed for oral DNA delivery

机译：用于口服DNA递送的生物粘附性氨基聚乙二醇化聚（酸酐）纳米颗粒的开发

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摘要

Amino-pegylated poly(methyl vinyl ether-co-maleic anhydride) nanoparticles were prepared applying a solvent displacement method. The surface charge of the resulting pegylated particles was considerably higher (-2.7 mV) than that of the non-pegylated (-33.5 mV). After oral administration to rats the amino-pegylated nanoparticles exhibited great ability for bioadhesive interactions with the gastrointestinal mucosa. Furthermore, fluorescence microscopy revealed that the amino-pegylated were able to cross the cellular membrane of the absorptive enterocytes. Genomic salmon testes DNA was associated to the amino-pegylated poly(anhydride) particles by applying two procedures: (i) incubation of aqueous DNA solution with the freshly formed amino-pegylated particles; and (ii) initial incubation of DNA and DAP-PEG simultaneously, followed by blending with preformed non-pegylated particles. Gel electrophoresis showed that both methods were safe and DNA integrity was not affected. Based on the results describing their adhesive properties and intracellular transport, the amino-pegylated nanoparticles were considered as a suitable carrier for DNA.
机译：应用溶剂置换法制备了氨基聚乙二醇化的聚（甲基乙烯基醚-马来酸酐共聚物）纳米粒子。所得聚乙二醇化颗粒的表面电荷（-2.7 mV）比未聚乙二醇化颗粒的表面电荷（-33.5 mV）高得多。对大鼠口服给药后，氨基聚乙二醇化的纳米颗粒表现出与胃肠道粘膜的生物粘附相互作用的巨大能力。此外，荧光显微镜显示氨基聚乙二醇化能够穿过吸收性肠细胞的细胞膜。基因组鲑鱼睾丸DNA通过以下两种方法与氨基聚乙二醇化的聚（酸酐）颗粒结合：（i）将DNA水溶液与新鲜形成的氨基聚乙二醇化的颗粒一起孵育; （ii）同时孵育DNA和DAP-PEG，然后与预先形成的未聚乙二醇化颗粒混合。凝胶电泳显示这两种方法都是安全的，并且DNA完整性没有受到影响。基于描述其粘附特性和细胞内运输的结果，氨基聚乙二醇化的纳米颗粒被认为是DNA的合适载体。

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