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首页> 外文期刊>Journal of Microencapsulation: Microcapsules Liposomes Nanoparticles Microcells Microspheres >DNA-chitosan nanoparticles improve DNA vaccine-elicited immunity against Newcastle disease virus through shuttling chicken interleukin-2 gene
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DNA-chitosan nanoparticles improve DNA vaccine-elicited immunity against Newcastle disease virus through shuttling chicken interleukin-2 gene

机译：DNA-壳聚糖纳米颗粒通过穿梭鸡白细胞介素2基因提高了DNA疫苗引发的针对新城疫病毒的免疫力

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摘要

In this study, pCAGG-ChlL2 plasmid DNA containing the chicken interleukin-2 (ChlL-2) gene was used to prepare DNA-chitosan nanoparticles (CNPs). The CNPs prepared were spherical, with mean diameters between 100 and 200 nm, have a positive surface charge, and could protect DNA against DNase I degradation. The CNPs prepared were successfully used to transfect the Df-1 cell line with almost no cytotoxicity. CNPs prepared at an amino group to phosphate group ratio (N/P ratio) of 16 provided the highest transfection efficiency (1.1 %) in medium with a pH of 6.5. When pCAGG-ChlL2 CNPs were administered to chickens simultaneously with a DNA vaccine against Newcastle disease virus (NDV), haemagglutination inhibition antibody titers and serum interferon-γ (IFN-γ) levels were significantly higher than in chickens immunised with the NDV DNA vaccine alone (p < 0.05). The results demonstrate that pCAGG-ChlL2 CNPs improve DNA vaccine-elicited immunity against NDV challenge.
机译：在这项研究中，包含鸡白介素2（ChlL-2）基因的pCAGG-ChlL2质粒DNA被用于制备DNA-壳聚糖纳米粒子（CNP）。制备的CNP为球形，平均直径在100至200 nm之间，具有正表面电荷，可以保护DNA免受DNase I降解。制备的CNP成功用于转染Df-1细胞系，几乎没有细胞毒性。以氨基与磷酸基团之比（N / P比）为16制备的CNP在pH为6.5的培养基中提供最高的转染效率（1.1％）。当将pCAGG-ChlL2 CNP与抗新城疫病毒（NDV）的DNA疫苗同时施用给鸡时，血凝抑制抗体滴度和血清干扰素-γ（IFN-γ）水平明显高于仅用NDV DNA疫苗免疫的鸡（p ＜0.05）。结果表明，pCAGG-ChlL2 CNP改善了DNA疫苗引发的抗NDV攻击的免疫力。

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