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首页> 外文期刊>Journal of Nematology, with Annual of Applied Nematology >Ribosomal DNA haplotype distribution of Bursaphelenchus xylophilus in Kyushu and Okinawa islands, Japan
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Ribosomal DNA haplotype distribution of Bursaphelenchus xylophilus in Kyushu and Okinawa islands, Japan

机译：日本九州岛和冲绳岛的Bursaphelenchus xylophilus核糖体DNA单倍型分布

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摘要

Ribosomal DNA region sequences (partial 18S, 28S and complete ITS1, 5.8S, and ITS2) of die pincwood nematode (Bursaphelenchus xylophilus) were obtained from DNA extracted directly from wood pieces collected from wilted pine trees throughout the Kyushuand Okinawa islands, Japan. Either a 2569bp or 2573bp sequence was obtained from 88 of 143 samples. Together with the 45 rDNA sequences of pinewood nematode isolates previously reported, there were eight single nucleotide polymorphisms and two indels oftwo bases. Based on these mutations, nine haplotypes were estimated. The haplotype frequencies differed among regions in Kyushu island (northwest, northeast and center, southeast, and southwest), and the distribution was consistent with the invasion andspreading routes of the pinewood nematode previously estimated from past records of pine wilt and wood importation. There was no significant difference in haplotype frequencies among the collection sites on Okinawa island.
机译：松材线虫（Bursaphelenchus xylophilus）的核糖体DNA区域序列（部分18S，28S和完整ITS1、5.8S和ITS2）是从直接从日本九州和冲绳群岛上枯萎的松树收集的木片中提取的DNA中获得的。从143个样品中的88个获得2569bp或2573bp的序列。连同先前报道的松木线虫分离株的45个rDNA序列，共有8个单核苷酸多态性和2个两个碱基的插入/缺失。基于这些突变，估计了九种单倍型。九州岛（西北部，东北部和中部，东南部和西南部）的区域之间的单倍型频率不同，其分布与先前根据松树枯萎和木材进口的先前记录估计的松材线虫的入侵和传播途径一致。在冲绳岛上的收集地点之间，单倍型频率没有显着差异。

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